Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
Clin Oral Investig. 2019 Feb;23(2):821-827. doi: 10.1007/s00784-018-2510-2. Epub 2018 Jun 6.
We aimed to evaluate molecular imaging as a novel diagnostic tool for mice periodontitis model induced by ligature and Porphyromonas gingivalis (Pg) inoculation.
Twelve female mice were assigned to the following groups: no treatment as control group (n = 4); periodontitis group induced by ligature and Pg as Pg group (n = 4); and Pg group treated with glycyrrhizinic acid (GA) as Pg + GA group (n = 4). All mice were administered a myeloperoxidase (MPO) activity-specific luminescent probe and observed using a charge-coupled device camera on day 14. Image analysis on all mice was conducted using software to determine the signal intensity of inflammation. Additionally, histological and radiographic evaluation for periodontal inflammation and bone resorption at the site of periodontitis, and quantitative enzyme-linked immunosorbent assay (ELISA) were conducted on three mice for each group. Each experiment was performed three times.
Levels of serum IgG antibody against P. gingivalis were significantly higher in the Pg than in the Pg + GA group. Histological analyses indicated that the number of osteoclasts and neutrophils were significantly lower in the Pg + GA than in the Pg group. Micro-CT image analysis indicated no difference in bone resorption between the Pg and Pg + GA groups. The signal intensity of MPO activity was detected on the complete craniofacial image; moreover, strong signal intensity was localized specifically at the periodontitis site in the ex vivo palate, with group-wise differences.
Molecular imaging analysis based on MPO activity showed high sensitivity of detection of periodontal inflammation in mice.
Molecular imaging analysis based on MPO activity has potential as a diagnostic tool for periodontitis.
评估分子成像作为一种新型诊断工具，用于结扎和牙龈卟啉单胞菌（Pg）接种诱导的小鼠牙周炎模型。
将 12 只雌性小鼠分为以下几组：不做任何处理作为对照组（n=4）；结扎和 Pg 诱导的牙周炎组作为 Pg 组（n=4）；Pg 组用甘草酸（GA）处理作为 Pg+GA 组（n=4）。所有小鼠均给予髓过氧化物酶（MPO）活性特异性发光探针，并于第 14 天使用电荷耦合器件（CCD）相机进行观察。使用软件对所有小鼠进行图像分析，以确定炎症信号强度。此外，对每组的 3 只小鼠进行牙周炎部位的牙周炎炎症和骨吸收的组织学和放射学评估，以及定量酶联免疫吸附试验（ELISA）。每个实验重复 3 次。
Pg 组血清 IgG 抗 P. gingivalis 抗体水平明显高于 Pg+GA 组。组织学分析表明，Pg+GA 组破骨细胞和中性粒细胞数量明显低于 Pg 组。Micro-CT 图像分析表明，Pg 组和 Pg+GA 组之间的骨吸收无差异。MPO 活性的信号强度在全颅面图像上被检测到；此外，在离体腭中，MPO 活性的信号强度在牙周炎部位特异性地强烈定位，且各组之间存在差异。
基于 MPO 活性的分子成像分析显示对小鼠牙周炎的检测具有较高的敏感性。
基于 MPO 活性的分子成像分析具有作为牙周炎诊断工具的潜力。
