López-Moya J J, Canto T, Díaz-Ruíz J R, López-Abella D
Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC, Velázquez, Madrid, Spain.
J Gen Virol. 1995 Sep;76 ( Pt 9):2293-7. doi: 10.1099/0022-1317-76-9-2293.
Two Spanish plum pox virus (PPV) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector Myzus persicae, with woody and herbaceous host plants. These isolates differ in the size of their coat protein (CP) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the N terminus of the CP, affecting the same positions as in a previously reported non-aphid-transmissible PPV isolate from Germany. Aphid transmission experiments showed that isolate 5.15 was transmitted from infected plants whereas isolate 3.3 was not. In contrast, both isolates were readily aphid-transmitted when acquired through artificial membranes from purified virus preparations supplemented with purified helper component (HC) obtained from potato virus Y-infected plants. This indicates that non-transmissibility of isolate 3.3 may be due to a defect in the HC rather than in the CP.
在涉及蚜虫介体桃蚜以及木本和草本寄主植物的传播实验中，使用了两种西班牙李痘病毒（PPV）分离株，5.15和3.3。这些分离株的外壳蛋白（CP）大小不同，序列分析显示，分离株3.3在CP的N端附近有15个氨基酸的缺失，与之前报道的一株来自德国的非蚜虫传播的PPV分离株受影响的位置相同。蚜虫传播实验表明，分离株5.15可从受感染植株传播，而分离株3.3则不能。相反，当从补充了从感染马铃薯Y病毒的植株中获得的纯化辅助成分（HC）的纯化病毒制剂通过人工膜获取时，两种分离株都很容易被蚜虫传播。这表明分离株3.3的不可传播性可能是由于HC存在缺陷而非CP。
