Goytia Elisa, Fernández-Calvino Lourdes, Martínez-García Belén, López-Abella Dionisio, López-Moya Juan José
Departamento de Biología de Plantas, Centro de Investigaciones Biológicas (CIB, CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.
Consorcio CSIC-IRTA, Instituto de Biología Molecular de Barcelona (IBMB, CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
J Gen Virol. 2006 Nov;87(Pt 11):3413-3423. doi: 10.1099/vir.0.82301-0.
Potyviruses are non-persistently transmitted by aphid vectors with the assistance of a viral accessory factor known as helper component (HC-Pro), a multifunctional protein that is also involved in many other essential processes during the virus infection cycle. A transient Agrobacterium-mediated expression system was used to produce Plum pox virus (PPV) HC-Pro in Nicotiana benthamiana leaves from constructs that incorporated the 5' region of the genome, yielding high levels of HC-Pro in agroinfiltrated leaves. The expressed PPV HC-Pro was able to assist aphid transmission of purified virus particles in a sequential feeding assay, and to complement transmission-defective variants of the virus. Also, HC-Pro of a second potyvirus, Tobacco etch virus (TEV), was expressed and found to be functional for aphid transmission. These results show that this transient system can be useful for production of functionally active HC-Pro in potyviruses, and the possible uses of this approach to study the mechanism of transmission are discussed.
马铃薯Y病毒属病毒借助一种名为辅助成分（HC-Pro）的病毒辅助因子由蚜虫介体进行非持久性传播，HC-Pro是一种多功能蛋白，在病毒感染周期的许多其他重要过程中也发挥作用。利用瞬时农杆菌介导的表达系统，通过整合基因组5'区域的构建体在本氏烟草叶片中产生李痘病毒（PPV）的HC-Pro，在农杆菌浸润的叶片中产生了高水平的HC-Pro。在连续取食试验中，表达的PPV HC-Pro能够协助蚜虫传播纯化的病毒粒子，并能互补病毒的传播缺陷变异体。此外，表达了第二种马铃薯Y病毒属病毒烟草蚀纹病毒（TEV）的HC-Pro，并发现其对蚜虫传播具有功能活性。这些结果表明，该瞬时系统可用于在马铃薯Y病毒属病毒中生产具有功能活性的HC-Pro，并讨论了该方法在研究传播机制方面的可能用途。
