Yu J, Fox J G, Blanco M C, Yan L, Correa P, Russell R M
Gastrointestinal Nutrition Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA.
J Nutr. 1995 Oct;125(10):2493-500. doi: 10.1093/jn/125.10.2493.
The effect of canthaxanthin (CX), a nonprovitamin A carotenoid, on gastric epithelial proliferation was studied in ferrets colonized with Helicobacter mustelae, which causes a chronic gastritis and an increased gastric epithelial cell proliferation. Seven spayed female ferrets were dosed by gavage with CX beadlets (50 mg/kg body wt, 5 d/wk) over 2 y, whereas seven control animals were given placebo beadlets. At the end of the 2-y-period, ferrets were killed, and gastric tissues were obtained from the antrum and body regions. A cell-proliferating biomarker, proliferating cell nuclear antigen (PCNA), was assayed by immunohistochemistry. The PCNA positivity was measured by a computer-based image analysis system. Serum concentrations of carotenoids, retinoids and tocopherols were analyzed by HPLC. Serum antioxidant status was measured by the oxygen radical absorbance capacity (ORAC) assay. The PCNA positivity in both antrum and body regions was not significantly different between CX-fed ferrets and controls. Serum CX concentrations were significantly higher in CX-fed ferrets vs. controls (P < 0.001), whereas levels of other carotenoids, retinoids and tocopherols were not significantly different. The ORAC values were not significant different between groups. This study does not suggest inhibitory effects of CX on gastric epithelial cell proliferation in H. mustelae infected ferrets.
研究了一种非维生素A类胡萝卜素角黄素（CX）对感染鼬源幽门螺杆菌的雪貂胃上皮细胞增殖的影响，该细菌可引起慢性胃炎并导致胃上皮细胞增殖增加。7只已绝育的雌性雪貂在2年时间里每周5天经口灌胃给予CX小丸（50毫克/千克体重），而另外7只对照动物给予安慰剂小丸。在2年实验期结束时，处死雪貂，获取胃窦和胃体组织。通过免疫组织化学检测细胞增殖生物标志物增殖细胞核抗原（PCNA）。采用基于计算机的图像分析系统测定PCNA阳性率。通过高效液相色谱法分析血清类胡萝卜素、视黄醇和生育酚的浓度。采用氧自由基吸收能力（ORAC）测定法检测血清抗氧化状态。给予CX的雪貂与对照组相比，胃窦和胃体区域的PCNA阳性率无显著差异。给予CX的雪貂血清CX浓度显著高于对照组（P < 0.001），而其他类胡萝卜素、视黄醇和生育酚的水平无显著差异。两组的ORAC值无显著差异。该研究未表明CX对感染鼬源幽门螺杆菌的雪貂胃上皮细胞增殖有抑制作用。
