用于检测375克混合样本的快速大肠杆菌O157:H7检测方法的灵敏度测定
Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples.
作者信息
Tsai W L, Miller C E, Richter E R
机构信息
Richter International, Inc., Columbus, Ohio 43201, USA.
出版信息
Appl Environ Microbiol. 2000 Sep;66(9):4149-51. doi: 10.1128/AEM.66.9.4149-4151.2000.
Abstract
Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g sample size.
摘要
采用免疫磁珠分离（IMS）技术与夹心酶联免疫吸附测定（ELISA）系统相结合的方法（IMS-ELISA），对25克单份绞碎牛肉样本和375克混合绞碎牛肉样本进行检测。结果表明，无论是25克还是375克样本量，IMS-ELISA均能检测到目标大肠杆菌O157:H7，检测限达每克样本10(-1) CFU 。
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