Konstantopoulou I, Ouzounis C A, Drosopoulou E, Yiangou M, Sideras P, Sander C, Scouras Z G
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, AUTH, Greece.
J Mol Evol. 1995 Oct;41(4):414-20. doi: 10.1007/BF00160312.
A clone isolated from a Drosophila auraria heat-shock cDNA library presents two long, antiparallel, coupled (LAC) open reading frames (ORFs). One strand ORF is 1,929 nucleotides long and exhibits great identity (87.5% at the nucleotide level and 94% at the amino acid level) with the hsp70 gene copies of D. melanogaster, while the second strand ORF, in antiparallel in-frame register arrangement, is 1,839 nucleotides long and exhibits 32% identity with a putative, recently identified, NAD(+)-dependent glutamate dehydrogenase (NAD(+)-GDH). The overlap of the two ORFs is 1,824 nucleotides long. Computational analysis shows that this LAC ORF arrangement is conserved in other hsp70 loci in a wide range of organisms, raising questions about possible evolutionary benefits of such a peculiar genomic organization.
从黑腹果蝇热休克cDNA文库中分离出的一个克隆呈现出两个长的、反平行且偶联的（LAC）开放阅读框（ORF）。一条链上的ORF长1929个核苷酸，与黑腹果蝇的hsp70基因拷贝具有高度同源性（核苷酸水平为87.5%，氨基酸水平为94%），而第二条链上的ORF以反平行框内排列方式存在，长1839个核苷酸，与一个推测的、最近鉴定出的NAD(+)依赖性谷氨酸脱氢酶（NAD(+)-GDH）具有32%的同源性。这两个ORF的重叠部分长1824个核苷酸。计算分析表明，这种LAC ORF排列在广泛生物体的其他hsp70基因座中是保守的，这引发了关于这种特殊基因组组织可能具有的进化益处的疑问。
