Merino E, Balbás P, Puente J L, Bolívar F
Departamento de Biología Molecular, Universidad Nacional Autónoma de Mexico, Cuernavaca.
Nucleic Acids Res. 1994 May 25;22(10):1903-8. doi: 10.1093/nar/22.10.1903.
Long Open Reading Frames (ORFs) in antisense DNA strands have been reported in the literature as being rare events. However, an extensive analysis of the GenBank database revealed that a substantial number of genes from several species contain an in-phase ORF in the antisense strand, that overlaps entirely the coding sequence of the sense strand, or even extends beyond. The findings described in this paper show that this is a frequent, non-random phenomenon, which is primarily dependent on codon usage, and to a lesser extent on gene size and GC content. Examination of the sequence database for several prokaryotic and eukaryotic organisms, demonstrates that coding sequences with in-phase, 100% overlapping antisense ORFs are present in every genome studied so far.
文献中报道，反义DNA链中的长开放阅读框（ORF）是罕见事件。然而，对GenBank数据库的广泛分析表明，来自多个物种的大量基因在反义链中含有一个同相位ORF，它与有义链的编码序列完全重叠，甚至延伸到其外。本文所述的研究结果表明，这是一种常见的、非随机的现象，主要取决于密码子使用情况，在较小程度上取决于基因大小和GC含量。对几种原核生物和真核生物的序列数据库进行检查表明，到目前为止，在所研究的每个基因组中都存在具有同相位、100%重叠反义ORF的编码序列。
