Lin J M, Lin C C, Chen M F, Ujiie T, Takada A
Graduate Institute of Natural Products, Kaohsiung Medical College, Taiwan, R.O.C.
J Ethnopharmacol. 1995 Jun;46(3):175-81. doi: 10.1016/0378-8741(95)01246-a.
The active oxygen species scavenging potencies of Mallotus repandus (Willd.) Muell.-Arg. extracts were evaluated by the electron spin resonance (ESR) spin-trapping technique. Superoxide radical (O2.-) and hydroxyl radical (OH.) were supplied enzymatically from hypoxanthine-xanthine oxidase (HPX-XOD) reaction and hydrogen peroxide-ferrous sulfate (Fenton reaction), respectively, to the assay system. The ethyl acetate fraction of Mallotus repandus (stem) showed the greatest superoxide radical scavenger activity and the n-hexane fraction of Mallotus repandus (stem as well as root) the greatest hydroxyl radical scavenger activity.
采用电子自旋共振（ESR）自旋捕获技术评估了白背叶提取物的活性氧清除能力。超氧阴离子自由基（O2.-）和羟基自由基（OH.）分别通过次黄嘌呤-黄嘌呤氧化酶（HPX-XOD）反应和过氧化氢-硫酸亚铁（芬顿反应）酶促产生，加入到检测体系中。白背叶（茎）的乙酸乙酯部位表现出最强的超氧阴离子自由基清除活性，而白背叶（茎和根）的正己烷部位表现出最强的羟基自由基清除活性。
