Hasan Md Mahadi, Uddin Nizam, Hasan Md Rakib, Islam A F M Mahmudul, Hossain Md Monir, Rahman Akib Bin, Hossain Md Sazzad, Chowdhury Ishtiaque Ahmed, Rana Md Sohel
Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
Department of Pharmacy, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
Biomed Res Int. 2014;2014:539807. doi: 10.1155/2014/539807. Epub 2014 Dec 31.
In folk medicine Mallotus repandus (Willd.) Muell. Arg. is used to treat muscle pain, itching, fever, rheumatic arthritis, snake bite, hepatitis, and liver cirrhosis. This study aimed to evaluate the antinociceptive as well as the anti-inflammatory activities of the methanol extract of leaf. The leaves were extracted with methanol following hot extraction and tested for the presence of phytochemical constituents. Analgesic and anti-inflammatory activities were evaluated using acetic acid induced writhing test, xylene induced ear edema, cotton pellet induced granuloma, and tail immersion methods at doses of 500, 1000, and 2000 mg/kg body weight. The presence of flavonoids, saponins, and tannins was identified in the extract. The extract exhibited considerable antinociceptive and anti-inflammatory activities against four classical models of pain. In acetic acid induced writhing, xylene induced ear edema, and cotton pellet granuloma models, the extract revealed dose dependent activity. Additionally, it increased latency time in tail immersion model. It can be concluded that M. repandus possesses significant antinociceptive potential. These findings suggest that this plant can be used as a potential source of new antinociceptive and anti-inflammatory candidates. The activity of methanol extract is most likely mediated through central and peripheral inhibitory mechanisms. This study justified the traditional use of leaf part of this plant.
在民间医学中，白背叶（Mallotus repandus (Willd.) Muell. Arg.）被用于治疗肌肉疼痛、瘙痒、发烧、风湿性关节炎、蛇咬伤、肝炎和肝硬化。本研究旨在评估叶甲醇提取物的抗伤害感受及抗炎活性。采用热提取法用甲醇提取叶片，并对植物化学成分的存在情况进行检测。以500、1000和2000毫克/千克体重的剂量，通过醋酸诱导扭体试验、二甲苯诱导耳肿胀、棉球诱导肉芽肿和尾部浸入法评估镇痛和抗炎活性。提取物中鉴定出了黄酮类、皂苷类和单宁类成分。该提取物对四种经典疼痛模型表现出相当的抗伤害感受和抗炎活性。在醋酸诱导扭体、二甲苯诱导耳肿胀和棉球肉芽肿模型中，提取物呈现出剂量依赖性活性。此外，它增加了尾部浸入模型中的潜伏时间。可以得出结论，白背叶具有显著的抗伤害感受潜力。这些发现表明，这种植物可作为新的抗伤害感受和抗炎候选物的潜在来源。甲醇提取物的活性很可能是通过中枢和外周抑制机制介导的。本研究证明了该植物叶部分的传统用途。
