Bosman G J, Steetzel B D, De Man A J, Van Kalmthout P J, Visser F E, De Grip W J
Department of Biochemistry, Faculty of Medicine, University of Nijmegen, The Netherlands.
Mech Ageing Dev. 1995 Apr 21;80(1):43-51. doi: 10.1016/0047-6374(94)01553-x.
Fluorescent microspheres were used to measure antibody-induced capping of leukocyte membrane proteins that are immunologically related to band 3, the anion exchanger of the erythrocyte. The degree of capping was found to increase with donor age. Surface labeling and capping characteristics of cells from healthy, age-matched controls were not different from those from patients with Alzheimer's disease, multi-infarct dementia, and Down's syndrome. Immunoblots, however, indicated increased expression and/or breakdown of band 3-like proteins in leukocytes from patients when compared with young and old control donors. These findings emphasize the possible involvement of band 3-like proteins of nucleated cells in aging and disease.
荧光微球被用于测量抗体诱导的白细胞膜蛋白帽化，这些蛋白在免疫学上与红细胞阴离子交换蛋白带3相关。发现帽化程度随供体年龄增加而升高。来自健康、年龄匹配对照组的细胞的表面标记和帽化特征与阿尔茨海默病、多发梗死性痴呆和唐氏综合征患者的细胞无异。然而，免疫印迹表明，与年轻和年老的对照供体相比，患者白细胞中带3样蛋白的表达增加和/或降解。这些发现强调了有核细胞的带3样蛋白可能参与衰老和疾病过程。
