Manninen P, Laakso P, Kallio H
Department of Biochemistry and Food Chemistry, University of Turku, Finland.
Lipids. 1995 Jul;30(7):665-71. doi: 10.1007/BF02537004.
The separation of gamma- and alpha-linolenic acid containing triacylglycerols with an identical acyl carbon number and degree of unsaturation was obtained on capillary supercritical fluid chromatography using a 25% cyanopropyl-75% methylpolysiloxane stationary phase. The resolution of 1,3-dioleoyl-2-alpha-linolenoyl-sn-glycerol was 1.35 on a 10 m x 50 microns i.d. column, whereas the resolution was enhanced to 1.66 by combining two 10-meter columns in series. The difference in the position of double bonds in one linolenic acid residue of triacylglycerols resulted in two series of peaks in the separation of alpine currant (Ribes alpinum) and black currant (R. nigrum) seed oils. The use of the 10-meter column was found to be appropriate for the screening of the triacylglycerol profile in both seed oils studied.
在毛细管超临界流体色谱上，使用25%氰丙基-75%甲基聚硅氧烷固定相，实现了具有相同酰基碳数和不饱和度的含γ-亚麻酸和α-亚麻酸的三酰甘油的分离。在一根10 m×50微米内径的色谱柱上，1,3-二油酰基-2-α-亚麻酰基-sn-甘油的分离度为1.35，而通过将两根10米长的色谱柱串联，分离度提高到了1.66。三酰甘油中一个亚麻酸残基双键位置的差异，导致了高山茶藨子（Ribes alpinum）和黑加仑（R. nigrum）籽油分离过程中出现两组峰。结果发现，10米长的色谱柱适用于所研究的两种籽油中三酰甘油谱的筛选。
