Christie W W
J Lipid Res. 1982 Sep;23(7):1072-5.
A simple procedure suitable for rapid transmethylation of triacylglycerols, other neutral lipids (including cholesteryl esters), and glycerophospholipids is described. Lipids in diethyl ether solution (50 volumes), in the presence of methyl acetate (1 vol), are reacted with 1 M sodium methoxide in methanol (1 vol) at room temperature. Essentially complete transmethylation can occur within a few minutes with no hydrolysis. Glassware and reagent requirements are minimal and samples are ready for gas-liquid chromatography analysis with very little work-up.
描述了一种适用于三酰甘油、其他中性脂质（包括胆固醇酯）和甘油磷脂快速甲基化的简单方法。在乙酸甲酯（1体积）存在下，二乙醚溶液（50体积）中的脂质在室温下与甲醇中的1 M甲醇钠（1体积）反应。几分钟内即可基本完全发生甲基化，且无水解现象。对玻璃器皿和试剂的要求极低，只需很少的后处理工作，样品即可用于气相色谱分析。
