Salas M L, Romero J F, Solarte Y, Olano V, Herrera M A, Herrera S
Fundación Centro de Primates, Cali, Colombia.
Mem Inst Oswaldo Cruz. 1994;89 Suppl 2:115-9. doi: 10.1590/s0074-02761994000600024.
The sporogonic cycle of Plasmodium vivax was established and maintained under laboratory conditions in two different strains of Anopheles albimanus mosquitoes using as a parasite source blood from human patients or from Aotus monkeys infected with the VCC-2 P.vivax colombian isolate. Both the Tecojate strain isolate from Guatemala and the Cartagena strain from the colombian Pacific coast were susceptible to infections with P.vivax. A higher percentage of Cartagena mosquitoes was infected per trial, however the Tecojate strain developed higher sporozoite loads. Intravenous inoculation of Aotus monkeys with sporozoites obtained from both anopheline strains resulted in successful blood infections. Animals infected with sporozoites from the Tecojate strain presented a patent period of 21-32 days whereas parasitemia appeared between days 19-53 in monkeys infected with sporozites from Cartagena strain.
间日疟原虫的孢子生殖周期在实验室条件下，使用来自人类患者或感染了间日疟原虫哥伦比亚分离株VCC - 2的夜猴的血液作为寄生虫来源，在两种不同品系的白纹伊蚊中得以建立和维持。来自危地马拉的特科哈特品系分离株和来自哥伦比亚太平洋沿岸的卡塔赫纳品系对白纹伊蚊均易感间日疟原虫感染。每次试验中，卡塔赫纳品系蚊子的感染比例更高，然而特科哈特品系产生的子孢子负荷更高。用从两种按蚊品系获得的子孢子对夜猴进行静脉接种，成功导致血液感染。感染特科哈特品系子孢子的动物出现虫血症的潜伏期为21 - 32天，而感染卡塔赫纳品系子孢子的猴子在第19 - 53天之间出现寄生虫血症。
