Roa307是一种由伯氏考克斯氏体质粒QpH1编码的蛋白质，与细菌染色体复制起始区域编码的蛋白质具有同源性。

Roa307, a protein encoded on Coxiella burnetii plasmid QpH1, shows homology to proteins encoded in the replication origin region of bacterial chromosomes.

作者信息

Lin Z, Howe D, Mallavia L P

机构信息

Department of Microbiology, Washington State University, Pullman 99164-4233, USA.

出版信息

Mol Gen Genet. 1995 Aug 30;248(4):487-90. doi: 10.1007/BF02191649.

DOI:10.1007/BF02191649

PMID:7565613

Abstract

TnphoA mutagenesis identified an open reading frame, roa307, immediately upstream of the partition locus qsopAB on the Coxiella burnetii plasmid QpH1. The protein sequence deduced from roa307 displayed homology to Orf290 of Pseudomonas putida, Orf283 and Orf282 (SpoOJ) of Bacillus subtilis-hypothetical products of genes in the chromosomal replication origin region. Expression of roa307 was demonstrated by PhoA activity of an Roa307-PhoA fusion.

摘要

TnphoA诱变鉴定出一个开放阅读框roa307，它紧邻柯克斯体（Coxiella burnetii）质粒QpH1上的分区位点qsopAB的上游。从roa307推导的蛋白质序列与恶臭假单胞菌（Pseudomonas putida）的Orf290、枯草芽孢杆菌（Bacillus subtilis）的Orf283和Orf282（SpoOJ）具有同源性，这些是染色体复制起始区域中基因的假定产物。Roa307-PhoA融合蛋白的PhoA活性证明了roa307的表达。