Thiele D, Willems H, Haas M, Krauss H
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Germany.
Eur J Epidemiol. 1994 Aug;10(4):413-20. doi: 10.1007/BF01719665.
The complete plasmid QpH1 from Coxiella burnetti, isolate 'Nine Mile', phase I, was cloned as NotI fragment with a size of 37329 bp. The entire plasmid was sequenced by the chain termination method after EcoRI subcloning. 37 open reading frames coding for polypeptides larger than 100 amino acid residues were determined. The predicted polypeptide products of the open reading frames were compared by computer analysis with reported protein sequences. Homologies of predicted polypeptide products to analogous proteins are described.
来自伯纳特柯克斯体“九英里”菌株I相的完整质粒QpH1,作为大小为37329 bp的NotI片段被克隆。在进行EcoRI亚克隆后,通过链终止法对整个质粒进行测序。确定了37个编码大于100个氨基酸残基的多肽的开放阅读框。通过计算机分析将开放阅读框的预测多肽产物与已报道的蛋白质序列进行比较。描述了预测多肽产物与类似蛋白质的同源性。