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A sporulation gene in Coxiella burnetii?

作者信息

Oswald W, Thiele D

机构信息

Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Germany.

出版信息

Zentralbl Veterinarmed B. 1993 Jul;40(5):366-70. doi: 10.1111/j.1439-0450.1993.tb00151.x.

Abstract

During a search for conserved target sequences on the genome of Coxiella burnetii for a diagnostic PCR a NotI/EcoRV DNA fragment was cloned and sequenced from the isolate "Nine Mile", Phase I (sequence data are registered under accession number X70045 in data bases EMBL, GENEBANK and DDBJ). On this fragment we have found a sequence of 1741 base pairs which showed an exceptionally high homology (60% on the nucleic acid level and 49.6% on the amino acid level) to the sporulation gene "spoIIIE" of the gram-positive bacterium Bacillus subtilis. This is first evidence of the molecular basis for formation of spores in Coxiella burnetii which has been postulated in the literature by electron microscopic investigations.

摘要

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