Helbig R, Speit G
Universität Ulm, Abteilung Medizinische Genetik, Germany.
Mutat Res. 1995 Sep;348(1):1-6. doi: 10.1016/0165-7992(95)90013-6.
The V-E5 cell line, a mutant V79 Chinese hamster cell line, was used to study the effect of chromosomal instability on the spectrum of gene mutations and chromosome aberrations induced by the anthracycline antibiotic adriamycin (AM). V-E5 cells showed hypersensitivity to the cytotoxic effects of AM when compared to the parental cell line. AM caused both, chromosome-type aberrations and chromatid-type aberrations in V-E5 cells. Under the same experimental conditions, gene mutations were induced at the hprt locus which mainly represented deletion mutations. The spectrum of AM-induced chromosomal aberrations and gene mutations did not show any peculiarities in comparison to normal V79 cells. It is concluded that the genomic instability in V-E5 cells does not influence the pathways leading to chromosome aberrations and gene mutations after AM treatment.
V-E5细胞系是一种突变的V79中国仓鼠细胞系，用于研究染色体不稳定性对蒽环类抗生素阿霉素（AM）诱导的基因突变谱和染色体畸变的影响。与亲代细胞系相比，V-E5细胞对AM的细胞毒性作用表现出超敏性。AM在V-E5细胞中引起了染色体型畸变和染色单体型畸变。在相同的实验条件下，在hprt基因座诱导了基因突变，主要表现为缺失突变。与正常V79细胞相比，AM诱导的染色体畸变和基因突变谱没有任何特殊之处。得出的结论是，V-E5细胞中的基因组不稳定性不影响AM处理后导致染色体畸变和基因突变的途径。
