利用随机扩增多态性DNA聚合酶链式反应鉴别拟似接骨木镰刀菌及相关物种
Differentiation of Fusarium sambucinum Fuckel sensu lato and related species by RAPD PCR.
作者信息
Hering O, Nirenberg H I
机构信息
Federal Biological Research Centre for Agriculture and Forestry, Institute of Microbiology, Berlin, Germany.
出版信息
Mycopathologia. 1995;129(3):159-64. doi: 10.1007/BF01103341.
DNA polymorphisms generated by the random amplified polymorphic DNA polymerase chain reaction (RAPD PCR) were used to analyse 41 isolates investigated in the European Fusarium sambucinum Project (EFSP). Employing ten arbitrary (10-mer) oigonucleotides and simple repeat sequences (M13, (GACA)4) as single primers, informative banding patterns typical for identifying European populations of Fusarium sambucinum Fuckel s. str., F. torulosum (Berk. & Curt.) Nirenberg and F. venenatum Nirenberg were obtained.
通过随机扩增多态性DNA聚合酶链反应（RAPD-PCR）产生的DNA多态性，用于分析欧洲接骨木镰孢菌项目（EFSP）中研究的41个分离株。使用10个任意（10聚体）寡核苷酸和简单重复序列（M13，(GACA)4）作为单引物，获得了用于鉴定欧洲接骨木镰孢菌 Fuckel s. str.、串珠镰孢菌（Berk. & Curt.）Nirenberg和尖孢镰孢菌Nirenberg群体的典型信息条带模式。
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