Goland R S, Conwell I M, Jozak S
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Placenta. 1995 Jun;16(4):375-82. doi: 10.1016/0143-4004(95)90095-0.
Prior studies have shown that levels of corticotrophin-releasing hormone (CRH) in the umbilical cord blood of infants born after pregnancies complicated by pre-eclampsia are significantly higher than fetal plasma CRH concentrations in uncomplicated pregnancies. In the present study we have measured CRH by radioimmunoassay in the placenta and fetal membranes from 13 pregnancies complicated by pre-eclampsia and compared them to 24 uncomplicated pregnancies. In addition we have investigated the effect of chronic intrauterine fetal stress on the processing of CRH in the placenta and fetal membranes. Placental CRH peptide content was significantly higher in the pregnancies complicated by pre-eclampsia, 12,900 +/- 4230 pg/g tissue, than in the uncomplicated pregnancies, 3130 +/- 430 pg/g of tissue (P < 0.01). Gel filtration of the homogenates of normal placenta revealed a major peak of CRH immunoactivity eluting in the same position as synthetic human CRH. A second smaller molecular weight peak of CRH-immunoactivity was also present and in both the amnion and the chorion, the CRH eluted in the position of the smaller molecular weight peak. In contrast, the bulk of the CRH immunoactivity in the placenta and fetal membranes obtained after pregnancies complicated by pre-eclampsia eluted in the position of intact synthetic human CRH. Thus, in pregnancy complicated by pre-eclampsia, both placental CRH release into fetal plasma and CRH peptide content is higher than in uncomplicated pregnancy.
先前的研究表明，子痫前期合并妊娠分娩的婴儿脐带血中促肾上腺皮质激素释放激素（CRH）水平显著高于正常妊娠胎儿血浆中的CRH浓度。在本研究中，我们采用放射免疫分析法测定了13例子痫前期合并妊娠的胎盘和胎膜中的CRH，并与24例正常妊娠进行比较。此外，我们还研究了慢性宫内胎儿应激对胎盘和胎膜中CRH加工过程的影响。子痫前期合并妊娠的胎盘CRH肽含量为12,900±4230 pg/g组织，显著高于正常妊娠的3130±430 pg/g组织（P<0.01）。正常胎盘匀浆的凝胶过滤显示，CRH免疫活性的一个主要峰与合成人CRH在同一位置洗脱。还存在一个分子量较小的CRH免疫活性峰，在羊膜和绒毛膜中，CRH在分子量较小的峰位置洗脱。相比之下，子痫前期合并妊娠后获得的胎盘和胎膜中的大部分CRH免疫活性在完整合成人CRH的位置洗脱。因此，子痫前期合并妊娠时，胎盘释放到胎儿血浆中的CRH和CRH肽含量均高于正常妊娠。
