Perkins A V, Linton E A, Eben F, Simpson J, Wolfe C D, Redman C W
Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, UK.
Br J Obstet Gynaecol. 1995 Feb;102(2):118-22. doi: 10.1111/j.1471-0528.1995.tb09063.x.
To measure the plasma levels of corticotrophin-releasing hormone and corticotrophin-releasing hormone binding protein in normal pregnancy and in pregnancies complicated by pre-eclampsia.
John Radcliffe Hospital, Oxford and St Thomas's Hospital, London.
One hundred and twenty pregnant women sampled prospectively throughout gestation, of whom 91 experienced a normal pregnancy and eight developed pre-eclampsia; in a second study, 10 women with severe pre-eclampsia, presenting at a range of gestational ages, were sampled once and compared with appropriately matched normal pregnant women.
Plasma levels of corticotrophin-releasing hormone determined by immunoradiometric assay. Plasma levels of corticotrophin-releasing hormone binding protein measured by direct radioimmunoassay.
In the prospective study, plasma samples from women with pre-eclampsia exhibited higher (390.2 versus 292.7 pmol/l at 36 weeks) levels of corticotrophin-releasing hormone and significantly lower (5.24 versus 8.14 nmol/l at 36 weeks, P < 0.002) levels of corticotrophin-releasing hormone binding protein than normal controls. In the second, single time point study a significant elevation in CRH (P < 0.002) and reduction in CRH-BP (P < 0.001) was found in pre-eclamptic pregnancies compared with controls.
In human pregnancies complicated by pre-eclampsia there is an elevated level of corticotrophin releasing hormone whilst there is less corticotrophin-releasing hormone binding protein; therefore there is a net increase in free potentially bioactive hormone which may play a role in the pathology of the disease.
测定正常妊娠及合并子痫前期妊娠的血浆促肾上腺皮质激素释放激素（CRH）和促肾上腺皮质激素释放激素结合蛋白（CRH-BP）水平。
牛津约翰拉德克利夫医院和伦敦圣托马斯医院。
120名孕妇在孕期进行前瞻性采样，其中91名经历正常妊娠，8名发生子痫前期；在第二项研究中，对10名不同孕周出现严重子痫前期的孕妇进行单次采样，并与匹配的正常孕妇进行比较。
采用免疫放射分析法测定血浆促肾上腺皮质激素释放激素水平。采用直接放射免疫分析法测定血浆促肾上腺皮质激素释放激素结合蛋白水平。
在前瞻性研究中，子痫前期孕妇的血浆样本中促肾上腺皮质激素释放激素水平较高（36周时为390.2 pmol/L，而正常对照组为292.7 pmol/L），促肾上腺皮质激素释放激素结合蛋白水平显著较低（36周时为5.24 nmol/L，正常对照组为8.14 nmol/L，P<0.002）。在第二项单时间点研究中，与对照组相比，子痫前期妊娠中促肾上腺皮质激素释放激素显著升高（P<0.002），促肾上腺皮质激素释放激素结合蛋白显著降低（P<0.001）。
在合并子痫前期的人类妊娠中，促肾上腺皮质激素释放激素水平升高，而促肾上腺皮质激素释放激素结合蛋白水平降低；因此，游离的潜在生物活性激素净增加，这可能在该疾病的病理过程中起作用。
