Yusibov V, Loesch-Fries L S
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 49706, USA.
Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8980-4. doi: 10.1073/pnas.92.19.8980.
A virus-based vector was used for the transient expression of the alfalfa mosaic virus coat protein (CP) gene in protoplasts and plants. The accumulation of wild-type CP conferred strong protection against subsequent alfalfa mosaic virus infection, enabling the efficacy of CP mutants to be determined without developing transgenic plants. Expression of the CP mRNA alone without CP accumulation conferred weaker protection against infection. The activity of the N-terminal mutant CPs in protection did not correlate with their activities in genome activation. The activity of a C-terminal mutant suggested that encapsidation did not have a role in protection. Our results indicate that interaction of the CP with alfalfa mosaic virus RNA is not important in protection, thereby leaving open the possibility that interactions with host factors lead to protection.
一种基于病毒的载体被用于在原生质体和植物中瞬时表达苜蓿花叶病毒外壳蛋白（CP）基因。野生型CP的积累赋予了对随后苜蓿花叶病毒感染的强大保护作用，使得无需培育转基因植物就能确定CP突变体的功效。仅CP mRNA的表达而无CP积累赋予的感染保护作用较弱。N端突变CP在保护中的活性与其在基因组激活中的活性不相关。一个C端突变体的活性表明衣壳化在保护中不起作用。我们的结果表明，CP与苜蓿花叶病毒RNA的相互作用在保护中并不重要，从而留下了与宿主因子的相互作用导致保护的可能性。
