Tenreiro S, Nobre M F, Hoste B, Gillis M, Kristjansson J K, da Costa M S
Departamento de Bioquímica, Universidade de Coimbra, Portugal.
Res Microbiol. 1995 May;146(4):315-24. doi: 10.1016/0923-2508(96)81054-0.
The species Thermus scotoductus was recently described as containing several non-pigmented isolates from Selfoss, Iceland, and the X-1 strain from the USA (Kristjansson et al., 1994). In this study, we performed DNA:DNA hybridizations and chemotaxonomic studies on several non-pigmented Thermus isolates from other geographical areas to assess their relationship to the strains originally assigned to this species. The results of DNA:DNA hybridizations showed that strains NH and Dl from London and strains Vl-7a and Vl-13 from Vizela, Portugal, belonged to T. scotoductus. T. scotoductus X-1 (ATCC 27978) was composed of two stable colony types, one of which had a major glycolipid different from the one present in the other colony type and from all other Thermus strains examined as well. The fatty acid composition of the isolates from Selfoss and London were practically identical. However, the fatty acid composition of strain X-1, the individual colony types of this strain and the Vizela strains were different from the Selfoss-London isolates and from each other. Another non-pigmented strain, designated SPS-11, belonged to a different DNA homology group.
嗜热栖热菌(Thermus scotoductus)这一物种最近被描述为包含来自冰岛塞尔福斯的几个无色素分离株以及来自美国的X-1菌株(克里斯蒂扬松等人,1994年)。在本研究中,我们对来自其他地理区域的几个无色素嗜热栖热菌分离株进行了DNA:DNA杂交和化学分类学研究,以评估它们与最初归为该物种的菌株之间的关系。DNA:DNA杂交结果表明,来自伦敦的NH和Dl菌株以及来自葡萄牙维泽拉的Vl-7a和Vl-13菌株属于嗜热栖热菌。嗜热栖热菌X-1(ATCC 27978)由两种稳定的菌落类型组成,其中一种的主要糖脂与另一种菌落类型以及所有其他检测的嗜热栖热菌菌株中的糖脂不同。来自塞尔福斯和伦敦的分离株的脂肪酸组成几乎相同。然而,X-1菌株、该菌株的各个菌落类型以及维泽拉菌株的脂肪酸组成与塞尔福斯 - 伦敦分离株不同,且彼此之间也不同。另一个无色素菌株,命名为SPS-11,属于一个不同的DNA同源组。