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嗜热栖热放线菌的DNA:DNA杂交及化学分类学研究

DNA:DNA hybridization and chemotaxonomic studies of Thermus scotoductus.

作者信息

Tenreiro S, Nobre M F, Hoste B, Gillis M, Kristjansson J K, da Costa M S

机构信息

Departamento de Bioquímica, Universidade de Coimbra, Portugal.

出版信息

Res Microbiol. 1995 May;146(4):315-24. doi: 10.1016/0923-2508(96)81054-0.

DOI:10.1016/0923-2508(96)81054-0
PMID:7569325
Abstract

The species Thermus scotoductus was recently described as containing several non-pigmented isolates from Selfoss, Iceland, and the X-1 strain from the USA (Kristjansson et al., 1994). In this study, we performed DNA:DNA hybridizations and chemotaxonomic studies on several non-pigmented Thermus isolates from other geographical areas to assess their relationship to the strains originally assigned to this species. The results of DNA:DNA hybridizations showed that strains NH and Dl from London and strains Vl-7a and Vl-13 from Vizela, Portugal, belonged to T. scotoductus. T. scotoductus X-1 (ATCC 27978) was composed of two stable colony types, one of which had a major glycolipid different from the one present in the other colony type and from all other Thermus strains examined as well. The fatty acid composition of the isolates from Selfoss and London were practically identical. However, the fatty acid composition of strain X-1, the individual colony types of this strain and the Vizela strains were different from the Selfoss-London isolates and from each other. Another non-pigmented strain, designated SPS-11, belonged to a different DNA homology group.

摘要

嗜热栖热菌(Thermus scotoductus)这一物种最近被描述为包含来自冰岛塞尔福斯的几个无色素分离株以及来自美国的X-1菌株(克里斯蒂扬松等人,1994年)。在本研究中,我们对来自其他地理区域的几个无色素嗜热栖热菌分离株进行了DNA:DNA杂交和化学分类学研究,以评估它们与最初归为该物种的菌株之间的关系。DNA:DNA杂交结果表明,来自伦敦的NH和Dl菌株以及来自葡萄牙维泽拉的Vl-7a和Vl-13菌株属于嗜热栖热菌。嗜热栖热菌X-1(ATCC 27978)由两种稳定的菌落类型组成,其中一种的主要糖脂与另一种菌落类型以及所有其他检测的嗜热栖热菌菌株中的糖脂不同。来自塞尔福斯和伦敦的分离株的脂肪酸组成几乎相同。然而,X-1菌株、该菌株的各个菌落类型以及维泽拉菌株的脂肪酸组成与塞尔福斯 - 伦敦分离株不同,且彼此之间也不同。另一个无色素菌株,命名为SPS-11,属于一个不同的DNA同源组。

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引用本文的文献

1
Identification of iron-reducing Thermus strains as Thermus scotoductus.将能还原铁的嗜热栖热菌菌株鉴定为暗产嗜热栖热菌。
Extremophiles. 2004 Feb;8(1):37-44. doi: 10.1007/s00792-003-0357-0. Epub 2003 Oct 11.
2
Characterization of novel long-chain 1,2-diols in Thermus species and demonstration that Thermus strains contain both glycerol-linked and diol-linked glycolipids.嗜热栖热菌属中新型长链1,2 -二醇的特性鉴定以及嗜热栖热菌菌株同时含有甘油连接型和二醇连接型糖脂的证明。
J Bacteriol. 1997 Oct;179(19):6154-62. doi: 10.1128/jb.179.19.6154-6162.1997.