Tenreiro S, Nobre M F, da Costa M S
Departamento de Bioquímica, Universidade de Coimbra, Portugal.
Int J Syst Bacteriol. 1995 Oct;45(4):633-9. doi: 10.1099/00207713-45-4-633.
Strains of Thermus silvanus sp. nov. and strains of Thermus chliarophilus sp. nov. were isolated from the hot spring at Vizela in northern Portugal and the hot spring at Alcafache in central Portugal, respectively. The strains of T. silvanus produce orange-red-pigmented colonies and have an optimum growth temperature of about 55 degrees C, while the strains of T. chliarophilus produce yellow-pigmented colonies and have an optimum growth temperature of about 50 degrees C. The strains of both species are catalase negative. These species can be distinguished from each other and from Thermus ruber by biochemical characteristics, fatty acid composition data, and 16S rRNA gene sequence data. Our phylogenetic analysis showed that strains VI-R2T (T = type strain) and ALT-8T belong to the T. ruber line of descent. The type strain of T. silvanus is strain VI-R2 (= DSM 9946), and the type strain of T. chliarophilus is strain ALT-8 (= DSM 9957).
嗜热栖热放线菌新种菌株和嗜热嗜氯放线菌新种菌株分别从葡萄牙北部维泽拉的温泉和葡萄牙中部阿尔卡法什的温泉中分离得到。嗜热栖热放线菌菌株产生橙红色菌落,最适生长温度约为55℃,而嗜热嗜氯放线菌菌株产生黄色菌落,最适生长温度约为50℃。这两个物种的菌株均过氧化氢酶阴性。通过生化特性、脂肪酸组成数据和16S rRNA基因序列数据,这些物种可以相互区分,也可与红栖热放线菌区分开来。我们的系统发育分析表明,菌株VI-R2T(T = 模式菌株)和ALT-8T属于红栖热放线菌的谱系。嗜热栖热放线菌的模式菌株是菌株VI-R2(= DSM 9946),嗜热嗜氯放线菌的模式菌株是菌株ALT-8(= DSM 9957)。