Heerwagen C, Pabst R, Westermann J
Center of Anatomy, Medical School of Hannover, Germany.
Scand J Immunol. 1995 Oct;42(4):480-6. doi: 10.1111/j.1365-3083.1995.tb03683.x.
Thoracic duct lymphocytes (TDL) continuously patrol through the body, facilitating immune responses at most sites. The neuropeptide Substance P might regulate immune responses by influencing the migration of TDL. Therefore, it was investigated whether Substance P affects the migration of thoracic duct B, T, CD8+ and CD4+ ('naive' and 'memory') lymphocytes from blood to lymph in vivo. Labelled TDL were either incubated with Substance P and then injected into normal rats, or incubated without Substance P and then injected into rats continuously receiving Substance P intravenously. The numbers of labeled B, T, CD8+ and CD4+ ('naive' and 'memory') lymphocytes were determined in blood and thoracic duct lymph for 1 and 5 days, respectively. Neither the in vitro incubation with Substance P nor its in vivo application influenced the disappearance of any lymphocyte subset from the blood or its reappearance in the lymph. In addition, continuous intravenous application of the Substance P antagonist CP 96.345 did not alter the volume or the lymphocyte number of the efferent lymph. The present study indicates that the nervous system does not influence immune responses via Substance P by altering the migration pattern of B, T, CD8+ and CD4+ ('naive' and 'memory') lymphocytes.
胸导管淋巴细胞（TDL）持续在体内循环，促进大多数部位的免疫反应。神经肽P物质可能通过影响TDL的迁移来调节免疫反应。因此，研究了P物质是否会影响体内胸导管B细胞、T细胞、CD8 +和CD4 +（“初始”和“记忆”）淋巴细胞从血液向淋巴的迁移。标记的TDL要么与P物质一起孵育，然后注射到正常大鼠体内，要么不与P物质孵育，然后注射到持续静脉注射P物质的大鼠体内。分别在第1天和第5天测定血液和胸导管淋巴中标记的B细胞、T细胞、CD8 +和CD4 +（“初始”和“记忆”）淋巴细胞的数量。体外与P物质孵育及其体内应用均未影响任何淋巴细胞亚群从血液中的消失或其在淋巴中的重新出现。此外，持续静脉注射P物质拮抗剂CP 96.345不会改变输出淋巴的体积或淋巴细胞数量。本研究表明，神经系统不会通过改变B细胞、T细胞、CD8 +和CD4 +（“初始”和“记忆”）淋巴细胞的迁移模式，经由P物质影响免疫反应。
