Westermann J, Matyas J, Persin S, van der Meide P, Heerwagen C, Pabst R
Center of Anatomy, Medical School of Hannover, Germany.
Scand J Immunol. 1994 Apr;39(4):395-402. doi: 10.1111/j.1365-3083.1994.tb03391.x.
The subset composition of the migrating lymphocyte pool is largely unknown. In order to determine the number of B, T, CD8+, CD4+ and CD4+ 'naive' (CD45RC+) and 'memory' (CD45RC-) lymphocytes in this pool, the thoracic duct lymph of the rat was drained for 7 days. The effect of lymphocyte depletion on the number of blood lymphocytes was also monitored. In addition, the influence of continuously applied interferon-gamma (IFN-gamma) on the mobilization of the migrating lymphocyte pool was investigated. Within 1 week 2 x 10(9) thoracic duct lymphocytes (TDL) were collected, which represents about 50% of the total lymphocyte pool of an adult rat. Among the migrating lymphocytes an early and a late mobilized population could be differentiated. In the former the CD4+ 'naive' (CD45RC+) T lymphocytes constituted the largest population, whereas in the latter it was the B lymphocytes. Continuous infusion of IFN-gamma did not affect the number of lymphocytes in the blood. In contrast, in the thoracic duct IFN-gamma reduced the appearance of all lymphocyte subsets. However, the pattern of reduction over time differed markedly depending on the population (early or late mobilized) and the phenotype (B- or T-lymphocyte subsets). Thus, the migrating lymphocyte pool of the rat is very heterogeneous regarding its populations and shows complex changes in the mobilization pattern after IFN-gamma stimulation. Future studies should focus on how the size and the composition of the migrating lymphocyte pool is regulated.
迁移淋巴细胞池的亚群组成在很大程度上尚不清楚。为了确定该池中B淋巴细胞、T淋巴细胞、CD8⁺淋巴细胞、CD4⁺淋巴细胞以及CD4⁺ “初始”（CD45RC⁺）和“记忆”（CD45RC⁻）淋巴细胞的数量，对大鼠的胸导管淋巴引流7天。同时监测淋巴细胞耗竭对血液淋巴细胞数量的影响。此外，还研究了持续应用干扰素-γ（IFN-γ）对迁移淋巴细胞池动员的影响。在1周内收集了2×10⁹个胸导管淋巴细胞（TDL），这约占成年大鼠总淋巴细胞池的50%。在迁移淋巴细胞中，可以区分出早期和晚期动员的群体。在早期动员群体中，CD4⁺ “初始”（CD45RC⁺）T淋巴细胞占最大比例，而在晚期动员群体中，B淋巴细胞占最大比例。持续输注IFN-γ不影响血液中淋巴细胞的数量。相反，在胸导管中，IFN-γ减少了所有淋巴细胞亚群的出现。然而，随着时间的推移，减少模式因群体（早期或晚期动员）和表型（B淋巴细胞或T淋巴细胞亚群）的不同而有显著差异。因此，大鼠的迁移淋巴细胞池在群体方面非常异质，并且在IFN-γ刺激后动员模式显示出复杂的变化。未来的研究应聚焦于迁移淋巴细胞池的大小和组成是如何被调节的。
