Hua J, Chang C, Sun Q, Meyerowitz E M
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Science. 1995 Sep 22;269(5231):1712-4. doi: 10.1126/science.7569898.
ERS (ethylene response sensor), a gene in the Arabidopsis thaliana ethylene hormone-response pathway, was uncovered by cross-hybridization with the Arabidopsis ETR1 gene. The deduced ERS protein has sequence similarity with the amino-terminal domain and putative histidine protein kinase domain of ETR1, but it does not have a receiver domain as found in ETR1. A missense mutation identical to the dominant etr1-4 mutation was introduced into the ERS gene. The altered ERS gene conferred dominant ethylene insensitivity to wild-type Arabidopsis. Double-mutant analysis indicates that ERS acts upstream of the CTR1 protein kinase gene in the ethylene-response pathway.
乙烯反应传感器（ERS）是拟南芥乙烯激素反应途径中的一个基因，通过与拟南芥ETR1基因交叉杂交而被发现。推导的ERS蛋白与ETR1的氨基末端结构域和假定的组氨酸蛋白激酶结构域具有序列相似性，但它没有ETR1中发现的受体结构域。将与显性etr1-4突变相同的错义突变引入ERS基因。改变后的ERS基因赋予野生型拟南芥显性乙烯不敏感性。双突变分析表明，ERS在乙烯反应途径中位于CTR1蛋白激酶基因的上游起作用。
