Schaller G E, Bleecker A B
Department of Botany, University of Wisconsin, Madison 53706, USA.
Science. 1995 Dec 15;270(5243):1809-11. doi: 10.1126/science.270.5243.1809.
Mutations in the ETR1 gene of Arabidopsis thaliana confer insensitivity to ethylene, which indicates a role for the gene product in ethylene signal transduction. Saturable binding sites for [14C]ethylene were detected in transgenic yeast expressing the ETR1 protein, whereas control yeast lacking ETR1 showed no detectable ethylene binding. Yeast expressing a mutant form of ETR1 (etr1-1) also showed no detectable ethylene binding, which provides an explanation for the ethylene-insensitive phenotype observed in plants carrying this mutation. Expression of truncated forms of ETR1 in yeast provided evidence that the amino-terminal hydrophobic domain of the protein is the site of ethylene binding. It was concluded from these results that ETR1 acts as an ethylene receptor in Arabidopsis.
拟南芥ETR1基因的突变导致对乙烯不敏感，这表明该基因产物在乙烯信号转导中起作用。在表达ETR1蛋白的转基因酵母中检测到了[14C]乙烯的饱和结合位点，而缺乏ETR1的对照酵母未检测到乙烯结合。表达ETR1突变形式（etr1-1）的酵母也未检测到乙烯结合，这为携带该突变的植物中观察到的乙烯不敏感表型提供了解释。在酵母中表达截短形式的ETR1提供了证据，证明该蛋白的氨基末端疏水结构域是乙烯结合位点。从这些结果得出结论，ETR1在拟南芥中作为乙烯受体起作用。
