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[利用高效液相色谱法通过尸体肝脏和肺中游离氨基酸含量测定死亡时间]

[The determination of the time of death by the content of free amino acids in the liver and lungs of cadavers using high-performance liquid chromatography].

作者信息

Mel'nikov Iu L, Alybaeva K N

出版信息

Sud Med Ekspert. 1995 Jul-Sep;38(3):10-3.

PMID:7570768
Abstract

High-performance liquid chromatography is proposed to be used for assessment of the time of death during a fortnight postmortem. The procedure consists in dynamic measurements of the concentrations of 20 free amino acids in cadaveric liver and lungs of subjects dead from mechanical injuries with or without ethanol in the blood at moderately high temperatures (18 to 23 C) and 40-60% humidity. A working diagram for determination of the time of death is offered and diagnostic microblocks consisting of 5 most informative and stable amino acids developed, permitting the diagnosis of the time of death with 95% reliability.

摘要

有人提议使用高效液相色谱法来评估死后两周内的死亡时间。该程序包括动态测量机械性损伤致死且血液中含有或不含有乙醇的受试者尸体肝脏和肺中20种游离氨基酸的浓度,测量条件为中等高温(18至23摄氏度)和40%-60%的湿度。给出了用于确定死亡时间的工作图表,并开发了由5种信息含量最高且最稳定的氨基酸组成的诊断微模块,能够以95%的可靠性诊断死亡时间。

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