Ureña P, Ferreira A, Kung V T, Morieux C, Simon P, Ang K S, Souberbielle J C, Segre G V, Drüeke T B, De Vernejoul M C
Departement de Nephrologie, INSERM Unité 90, Hôpital Necker-Enfants Malades, Paris, France.
J Bone Miner Res. 1995 Jun;10(6):932-9. doi: 10.1002/jbmr.5650100614.
Type I collagen represents more than 90% of bone matrix. Quantitative analysis of collagen cross-link molecules such as pyridinoline (PYD) provides valuable information on bone resorption rate. We have studied 37 hemodialysis patients who underwent a systematic transiliac bone biopsy for histomorphometry study. Eighteen of them had tetracycline double labeling, allowing to determine dynamic, in addition to static bone parameters. Measurement of serum-free PYD was performed using a new competitive enzyme immunoassay. Serum PYD values were compared with those of three other serum markers of bone metabolism, namely intact PTH (iPTH), bone-specific alkaline phosphatase (bAP), and osteocalcin, for the correlations with bone histomorphometric parameters. Serum PYD levels (mean +/- SD) were significantly higher in dialysis patients than in normal individuals, 90.6 +/- 99.6 nM versus 1.9 +/- 0.4 nM, respectively. Patients with high turnover bone disease had significantly higher serum PYD levels than patients with normal or low bone turnover, 108.8 +/- 108.0 nM versus 34.1 +/- 12.8 nM, respectively. Serum PYD levels were positively correlated with bone resorption parameters including osteoclast surface (r = 0.59, p < 0.0001) and osteoclast number/mm2 (r = 0.61, p < 0.0001), and also with bone formation parameters, osteoblast surface (r = 0.43, p < 0.008), double-labeled surface (r = 0.81, p < 0.001), and BFR (r = 0.91, p < 0.0001). The BFR was better correlated with serum PYD levels than with either serum iPTH or osteocalcin concentrations. However, correlation with serum bAP was comparable.(ABSTRACT TRUNCATED AT 250 WORDS)
I型胶原蛋白占骨基质的90%以上。对诸如吡啶啉（PYD）等胶原交联分子进行定量分析可提供有关骨吸收速率的有价值信息。我们研究了37例接受系统性髂骨活检以进行组织形态计量学研究的血液透析患者。其中18例进行了四环素双标记，除了静态骨参数外，还可确定动态骨参数。使用一种新的竞争性酶免疫测定法测量血清游离PYD。将血清PYD值与其他三种骨代谢血清标志物，即完整甲状旁腺激素（iPTH）、骨特异性碱性磷酸酶（bAP）和骨钙素的值进行比较，以研究它们与骨组织形态计量学参数的相关性。透析患者的血清PYD水平（平均值±标准差）显著高于正常个体，分别为90.6±99.6 nM和1.9±0.4 nM。高转换型骨病患者的血清PYD水平显著高于骨转换正常或低的患者，分别为108.8±108.0 nM和34.1±12.8 nM。血清PYD水平与包括破骨细胞表面（r = 0.59，p < 0.0001）和破骨细胞数量/mm2（r = 0.61，p < 0.0001）在内的骨吸收参数呈正相关，也与骨形成参数、成骨细胞表面（r = 0.43，p < 0.008）、双标记表面（r = 0.81，p < 0.001）和骨形成率（BFR）（r = 0.91，p < 0.0001）呈正相关。与血清iPTH或骨钙素浓度相比，BFR与血清PYD水平的相关性更好。然而，与血清bAP的相关性相当。（摘要截断于250字）
