Eriksen E F, Charles P, Melsen F, Mosekilde L, Risteli L, Risteli J
University Department of Endocrinology and Metabolism, Aarhus Amtssygehus, Denmark.
J Bone Miner Res. 1993 Feb;8(2):127-32. doi: 10.1002/jbmr.5650080202.
Type I collagen makes up more than 90% of bone matrix. Therefore, analysis of antigens related to collagen formation and degradation in bone should provide good and specific estimates of both bone resorption and bone formation rates. In this study we measured serum levels of the pyridinoline cross-linked telopeptide domain of type I collagen (ICTP) as a marker of bone resorption and serum carboxy-terminal propeptide of type I procollagen (PICP) as a marker of bone formation. Serum levels of the two antigens were correlated to histomorphometric indices of bone resorption and bone formation calculated from iliac crest bone biopsies in a group of 18 individuals with high- and low-turnover bone disease (myxedema, primary hyperparathyroidism, and thyrotoxicosis). After logarithmic transformation the regression of S-ICTP on volume-referent resorption rate (BRs/R/BV) was significant (r = 0.61, p < 0.01, SEM/Y = 56%). S-ICTP also showed a significant regression on the volume-referent cancellous bone balance (r = -0.45, p < 0.05, SEM/Y = 412%). S-PICP was significantly correlated to the mineral appositional rate (r = 0.53, p < 0.05) and volume-referent bone formation rate (r = 0.61, p < 0.01, SEM/Y = 48%). The correlation to bone turnover as expressed in the activation frequency was also highly significant (r = 0.61, p < 0.01, SEM/Y = 51%). No significant correlation with wall thickness or bone balance was demonstrable per remodeling cycle. Thus, assays employing antigens that reflect collagen formation and degradation are useful instruments for the evaluation of rates of bone remodeling in metabolic bone disease.
I型胶原蛋白占骨基质的90%以上。因此，分析与骨中胶原蛋白形成和降解相关的抗原，应该能够很好地、特异性地评估骨吸收和骨形成速率。在本研究中，我们测量了I型胶原蛋白吡啶啉交联端肽结构域（ICTP）的血清水平作为骨吸收的标志物，以及I型前胶原羧基末端前肽（PICP）的血清水平作为骨形成的标志物。在一组18例高转换和低转换骨病（黏液水肿、原发性甲状旁腺功能亢进和甲状腺毒症）患者中，将这两种抗原的血清水平与通过髂嵴骨活检计算出的骨吸收和骨形成的组织形态计量学指标进行了关联。经对数转换后，S-ICTP与体积参照吸收速率（BRs/R/BV）的回归具有显著性（r = 0.61，p < 0.01，SEM/Y = 56%）。S-ICTP与体积参照松质骨平衡也呈现出显著的回归关系（r = -0.45，p < 0.05，SEM/Y = 412%）。S-PICP与矿物质沉积率显著相关（r = 0.53，p < 0.05），与体积参照骨形成率也显著相关（r = 0.61，p < 0.01，SEM/Y = 48%）。与以激活频率表示的骨转换的相关性也非常显著（r = 0.61，p < 0.01，SEM/Y = 51%）。在每个重塑周期中，未发现与壁厚度或骨平衡有显著相关性。因此，采用反映胶原蛋白形成和降解的抗原的检测方法，是评估代谢性骨病中骨重塑速率的有用工具。
