Corwin R L, Rowe P M, Crawley J N
Section of Behavioral Neuropharmacology, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892, USA.
Am J Physiol. 1995 Sep;269(3 Pt 2):R511-8. doi: 10.1152/ajpregu.1995.269.3.R511.
The neuropeptide galanin has been proposed to play a role in the regulation of fat intake. The purpose of the present investigation was to determine if galanin and the galanin receptor antagonist M40 would have selective effects on fat intake in a fat-chow choice paradigm in rats. Rats were adapted to 22-h access to chow alone and 2-h daily access to separate sources of fat and chow in the early dark cycle. Galanin (300 pmol, 1 nmol) or M40 (2-500 pmol) was microinjected bilaterally into the paraventricular nucleus of the hypothalamus (PVN) before the 2-h choice period, and chow and fat intake were measured. M40 had no effect on chow or fat intake. Galanin stimulated chow intake and increased the ratio of chow to fat consumed but had no significant effect on fat intake alone. These results suggest that endogenous galanin in the PVN may not play a primary role in the regulation of fat intake when fat is available in addition to a nutritionally balanced diet.
神经肽甘丙肽被认为在脂肪摄入调节中发挥作用。本研究的目的是确定甘丙肽和甘丙肽受体拮抗剂M40在大鼠脂肪-普通饲料选择模式下是否对脂肪摄入有选择性影响。大鼠在黑暗周期早期适应仅22小时可获取普通饲料，以及每天2小时可获取单独的脂肪和普通饲料来源。在2小时选择期前，将甘丙肽（300皮摩尔、1纳摩尔）或M40（2 - 500皮摩尔）双侧微量注射到下丘脑室旁核（PVN），并测量普通饲料和脂肪摄入量。M40对普通饲料或脂肪摄入没有影响。甘丙肽刺激普通饲料摄入并增加所消耗的普通饲料与脂肪的比例，但对单独的脂肪摄入没有显著影响。这些结果表明，当除了营养均衡的饮食外还有脂肪时，PVN中的内源性甘丙肽可能在脂肪摄入调节中不发挥主要作用。
