Hewitt C W, Englesbe M J, Tatem L D, Strande L F, Doolin E J, Dalsey R M, DeLong W G
Department of Surgery, UMDNJ-Robert Wood Johnson Medical School, Cooper Hospital/University Medical Center, Camden 08103, USA.
Ann Plast Surg. 1995 Jul;35(1):108-12. doi: 10.1097/00000637-199507000-00021.
The development of either unstable immune chimerism and lethal graft-versus-host disease or stable immune chimerism and alloimmune tolerance can result from extremity transplantation. LBN rats served as recipients of Lewis vascularized extremity (limb) transplants. Recipients received no immune suppression and were immunologically unmodified. The bone marrow of transplanted and contralateral limbs was analyzed in situ for distribution of nuclei, nuclear area, and staining intensity by digital image analysis and computerized morphometry. Cellularity was significantly increased, and fat content was significantly decreased in the graft-versus-host disease animals' marrow versus the tolerant animals' marrow for both the transplanted and contralateral limbs. Tolerant animals demonstrated significantly increased nuclear staining compared with graft-versus-host disease animals for both transplanted and contralateral limbs. Additionally, there were significant changes between the host and the transplanted limbs for marrow intensity and cellularity within tolerant and graft-versus-host disease groups. The significant differences in the graft-versus-host disease-positive recipients suggested that both autoimmune dysregulation and alloimmune reactions were in effect for both donor and host bone marrow compartments. Cellular alterations in the tolerant recipients' marrow were suggestive of subtle subclinical graft-versus-host responses.
肢体移植可能导致不稳定免疫嵌合体和致死性移植物抗宿主病的发生，也可能导致稳定免疫嵌合体和同种免疫耐受的形成。LBN大鼠作为Lewis带血管化肢体移植的受体。受体未接受免疫抑制，免疫状态未改变。通过数字图像分析和计算机形态测量法，对移植肢体和对侧肢体的骨髓进行原位分析，观察细胞核分布、核面积和染色强度。与耐受动物的骨髓相比，移植物抗宿主病动物移植肢体和对侧肢体的骨髓细胞密度显著增加，脂肪含量显著降低。与移植物抗宿主病动物相比，耐受动物移植肢体和对侧肢体的细胞核染色均显著增强。此外，在耐受组和移植物抗宿主病组中，宿主和移植肢体的骨髓强度和细胞密度也存在显著差异。移植物抗宿主病阳性受体的显著差异表明，自身免疫失调和同种免疫反应在供体和宿主骨髓区室中均起作用。耐受受体骨髓中的细胞改变提示存在轻微的亚临床移植物抗宿主反应。
