Furutani M, Arii S, Higashitsuji H, Mise M, Fukumoto M, Takano S, Nakayama H, Imamura M, Fujita J
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Biochem J. 1995 Oct 1;311 ( Pt 1)(Pt 1):203-8. doi: 10.1042/bj3110203.
We isolated a cDNA clone, kan-1, from a rat liver cDNA library using a reverse transcriptase PCR cloning method. The kan-1 cDNA encoded a polypeptide of 420 amino acids, and was 70 and 69% identical in nucleotide and amino acid sequences respectively with human liver bile acid-CoA-amino acid N-acyltransferase (BAT). Thus Kan-1 is probably a rat homologue of human BAT (rBAT). Kan-1/rBAT mRNA was mainly expressed in the livers of adult rats and rats immediately after, but not before, birth. It was expressed in the hepatocytes, the sinusoidal endothelial cells and the Kupffer cells of the liver. An anti-Kan-1/rBAT polyclonal antibody detected a protein of molecular mass 46 kDa in the liver. After partial hepatectomy, the levels of Kan-1/rBAT mRNA decreased at 6 and 12 h in the regenerating liver. In a sepsis model, hepatic expression of Kan-1/rBAT mRNA decreased at 6 and 12 h after caecal ligation and puncture. The kinetics of Kan-1/rBAT mRNA expression suggests that it may play a role in acute-phase reactions.
我们采用逆转录酶聚合酶链反应克隆方法，从大鼠肝脏cDNA文库中分离出一个cDNA克隆kan-1。kan-1 cDNA编码一个由420个氨基酸组成的多肽，其核苷酸序列和氨基酸序列与人肝脏胆汁酸辅酶A-氨基酸N-酰基转移酶（BAT）的一致性分别为70%和69%。因此，Kan-1可能是人类BAT（rBAT）的大鼠同源物。Kan-1/rBAT mRNA主要在成年大鼠肝脏以及出生后而非出生前的大鼠肝脏中表达。它在肝脏的肝细胞、窦状内皮细胞和枯否细胞中表达。一种抗Kan-1/rBAT多克隆抗体在肝脏中检测到一种分子量为46 kDa的蛋白质。部分肝切除术后，再生肝脏中Kan-1/rBAT mRNA水平在6小时和12小时时下降。在脓毒症模型中，盲肠结扎和穿刺后6小时和12小时，肝脏中Kan-1/rBAT mRNA的表达下降。Kan-1/rBAT mRNA表达的动力学表明它可能在急性期反应中发挥作用。
