He W, Sun H, Yang B, Zhang D, Kabelitz D
Institute of Immunology, University of Heidelberg, Germany.
Arzneimittelforschung. 1995 Aug;45(8):910-3.
The in vitro effects of the Chinese Herba Epimediia glycoside icariine (ICA), a chinese herbal extract, on human immune responses were investigated. ICA induced a weak and delayed proliferation of peripheral blood mononuclear cells from healthy donors when compared to phytohemagglutinin. Both T- (TCR alpha beta+) and B cells were the ICA-responding cells. Within T-cells, the relative proportion of CD4-8+ cells increased but that of CD4+8- cells decreased. ICA in certain concentrations could increase lymphokine-activated killer cell (LAK) activity (0.1 to 1.0 microgram/ml) in both tumor patients and healthy donors, and natural killer cell (NK) activity (1.0 to 5.0 micrograms/ml) in tumor patients. Moreover, ICA stimulated the production of tumor necrosis factor-alpha in monocytes from healthy donors. These findings provide evidence that ICA could be applied to adoptive immunotherapy. Generation of LAK cells in presence of an appropriate dose of ICA might be superior to interleukin-2 alone.
研究了中国草药提取物淫羊藿苷（ICA）对人体免疫反应的体外作用。与植物血凝素相比，ICA诱导健康供体外周血单个核细胞增殖作用较弱且延迟。T细胞（TCRαβ +）和B细胞均为对ICA产生反应的细胞。在T细胞内，CD4 - 8 +细胞的相对比例增加，而CD4 + 8 - 细胞的相对比例降低。一定浓度的ICA可增强肿瘤患者和健康供体的淋巴因子激活的杀伤细胞（LAK）活性（0.1至1.0微克/毫升），以及肿瘤患者的自然杀伤细胞（NK）活性（1.0至5.0微克/毫升）。此外，ICA刺激健康供体单核细胞产生肿瘤坏死因子-α。这些发现证明ICA可应用于过继性免疫治疗。在适当剂量的ICA存在下生成LAK细胞可能优于单独使用白细胞介素-2。
