Grohmann U, Fioretti M C, Binaglia L, Belladonna M L, Bianchi R, Puccetti P
University of Perugia, Department of Experimental Medicine and Biochemical Sciences, Italy.
Int J Cancer. 1993 Sep 9;55(2):344-50. doi: 10.1002/ijc.2910550227.
A triazene-xenogenized tumor sub-line was derived from the mouse mastocytoma cell line P815 following several transplant generations in vivo on DTIC. The highly immunogenic P815/DTIC variant line expressed new CTL-defined antigens. Novel antigens were also detected by antibodies in immunoprecipitation and by Western blot analysis. Upon immunoprecipitation of metabolically labeled cells, one such variant-specific 20-kDa antigen was shown to be related to retroviral envelope protein p15E. When injected intrasplenically into recipient mice, the electroblotted nitrocellulose-bound 20-kDa antigen resulted in increased frequency in CTL precursors to P815/DTIC cells. In addition to previous data in the L5178Y/DTIC tumor-model system, these data suggest that expression of aberrant, retrovirus-related proteins may be a common finding in different parental tumors xenogenized by triazene treatment.
在体内经数次氮烯咪胺移植传代后，从小鼠肥大细胞瘤细胞系P815衍生出一种三氮烯异种移植肿瘤亚系。高度免疫原性的P815/DTIC变异株表达了新的CTL定义抗原。通过免疫沉淀中的抗体以及蛋白质印迹分析也检测到了新抗原。对代谢标记细胞进行免疫沉淀时，一种这样的变异株特异性20 kDa抗原显示与逆转录病毒包膜蛋白p15E有关。当将电印迹的硝酸纤维素结合的20 kDa抗原脾内注射到受体小鼠中时，导致针对P815/DTIC细胞的CTL前体细胞频率增加。除了之前在L5178Y/DTIC肿瘤模型系统中的数据外，这些数据表明，异常的、逆转录病毒相关蛋白的表达可能是三氮烯处理异种移植的不同亲本肿瘤中的常见现象。
