Fava F, Armenante P M, Kafkewitz D
Department of Applied Chemistry and Material Science, Università di Bologna, Italy.
Lett Appl Microbiol. 1995 Nov;21(5):307-12. doi: 10.1111/j.1472-765x.1995.tb01066.x.
A Gram-negative aerobic bacterium capable of using 2-chlorophenol (2-CP), 3-chlorophenol (3-CP) and 4-chlorophenol (4-CP) as sole carbon sources was isolated and characterized. The bacterium, designated LD1, was identified to be a Pseudomonas pickettii strain. LD1 was able to totally degrade and dechlorinate 2-CP (initial concentration: 1.51 mmol l-1), 3-CP (initial concentration: 0.57 mmol l-1) and 4-CP (initial concentration: 0.75 mmol l-1) within 30, 30 and 40 h of incubation, respectively, under growing-cell batch conditions. LD1 was also found to be able to metabolize chlorocatechols in growing- and resting-cell conditions. This suggests that the bacterium degrades monochlorophenols through a chlorocatechol pathway. In addition, LD1 was found to be capable of readily metabolizing other organic compounds such as phenol, benzoic acid, hydroxybenzoic acids and hydroquinone. Because of the broad spectrum of monochlorophenols and organic compounds that LD1 can degrade, this bacterium appears to have the potential for being successfully used in the biotreatment of wastewaters and in soil decontamination.
分离并鉴定了一种革兰氏阴性需氧细菌，该细菌能够将2-氯苯酚（2-CP）、3-氯苯酚（3-CP）和4-氯苯酚（4-CP）作为唯一碳源。该细菌命名为LD1，被鉴定为皮氏假单胞菌菌株。在生长细胞分批培养条件下，LD1能够在30、30和40小时内分别将初始浓度为1.51 mmol l-1的2-CP、初始浓度为0.57 mmol l-1的3-CP和初始浓度为0.75 mmol l-1的4-CP完全降解并脱氯。还发现LD1在生长细胞和静止细胞条件下都能够代谢氯代儿茶酚。这表明该细菌通过氯代儿茶酚途径降解单氯苯酚。此外，发现LD1能够轻易代谢其他有机化合物，如苯酚、苯甲酸、羟基苯甲酸和对苯二酚。由于LD1能够降解的单氯苯酚和有机化合物种类广泛，该细菌似乎有潜力成功用于废水的生物处理和土壤净化。
