Kafkewitz D, Fava F, Armenante P M
Department of Biological Science, Rutgers University, Newark, NJ 07102, USA.
Appl Microbiol Biotechnol. 1996 Nov;46(4):414-21. doi: 10.1007/BF00166239.
The effect of vitamins on the aerobic degradation and dechlorination of 2-chlorophenol and 4-chlorophenol by Pseudomonas pickettii, strain LD1, and 4-chlorobiphenyl by Pseudomonas sp. strain CPE1 was determined. These microorganisms are capable of using the target compounds as the sole carbon and energy source, but do not need vitamins to metabolize them. The addition to the culture medium of a vitamin solution containing biotin, folic acid, pyridoxine hydrochloride, riboflavin, thiamine hydrochloride, niacin, pantothenic acid, cyanocobalamin, p-aminobenzoic acid, and thioctic acid (total final concentration: < or = 600 ppb) resulted in a 7%-16% increase in the amount of target compounds degraded over the incubation period required for the concentration of the compound in the cultures to drop to approximately zero. A corresponding increase in the amount of chloride ion produced was also detected during the same period, indicating active (and often stoichiometric) dechlorination of the target compounds.
测定了维生素对皮氏假单胞菌LD1菌株对2-氯苯酚和4-氯苯酚以及假单胞菌属CPE1菌株对4-氯联苯的好氧降解和脱氯作用的影响。这些微生物能够利用目标化合物作为唯一的碳源和能源，但代谢这些化合物不需要维生素。向培养基中添加含有生物素、叶酸、盐酸吡哆醇、核黄素、盐酸硫胺素、烟酸、泛酸、氰钴胺素、对氨基苯甲酸和硫辛酸的维生素溶液（最终总浓度：≤600 ppb），在培养物中化合物浓度降至约零所需的孵育期内，目标化合物降解量增加了7%-16%。在同一时期还检测到产生的氯离子量相应增加，表明目标化合物发生了活性（且通常是化学计量的）脱氯。
