Tsourvakas S, Hatzigrigoris P, Tsibinos A, Kanellakopoulou K, Giamarellou H, Dounis E
Orthopaedic Department, Laiko General Hospital, Athens, Greece.
Arch Orthop Trauma Surg. 1995;114(5):295-7. doi: 10.1007/BF00452091.
We prepared a composite of fibrin clot and ciprofloxacin for use as a biodegradable antibiotic delivery system with sustained effect for the treatment of chronic osteomyelitis. In vitro, ten experiments were performed in which 10 mg of ciprofloxacin were incorporated into 4 ml of fibrin clot. The clots were preserved in nutrient broth and incubated at 37 degrees C for a total of 60 days. Every 24 h a broth specimen was obtained, and the ciprofloxacin concentration was determined by microbiological assay. The maximum level of antibiotic was noted on the first day (49.9 +/- 5.1 mg/l). The ciprofloxacin-fibrin clot complexes usually disintegrated after 60 days. In vivo, the fibrin-ciprofloxacin clots were made as previously described. The composite was implanted in the medullary canal of rabbit tibiae, and the antibiotic concentration was measured in bone, muscle, skin and serum. In all tissues around the implant, the concentration of antibiotic exceeded the minimum inhibitory concentration against the common causative organisms of osteomyelitis for 10 days. The implant caused no systemic side-effects, and it is likely to prove clinically useful as a drug delivery system for treating chronic osteomyelitis.
我们制备了一种含有纤维蛋白凝块和环丙沙星的复合物，用作具有持续效果的可生物降解抗生素递送系统，用于治疗慢性骨髓炎。在体外，进行了十项实验，将10毫克环丙沙星加入4毫升纤维蛋白凝块中。将凝块保存在营养肉汤中，于37摄氏度孵育60天。每24小时获取一份肉汤样本，并通过微生物测定法测定环丙沙星浓度。第一天观察到抗生素的最高水平（49.9±5.1毫克/升）。环丙沙星-纤维蛋白凝块复合物通常在60天后解体。在体内，按照先前描述的方法制备纤维蛋白-环丙沙星凝块。将该复合物植入兔胫骨的髓腔内，并测定骨、肌肉、皮肤和血清中的抗生素浓度。在植入物周围的所有组织中，抗生素浓度超过针对骨髓炎常见致病菌的最低抑菌浓度达10天。该植入物未引起全身副作用，并且作为治疗慢性骨髓炎的药物递送系统可能在临床上证明是有用的。
