Zhao K S, Wang Y F, Guéret R, Weksler M E
Division of Geriatrics and Gerontology, Cornell University Medical College, New York 10021, USA.
Int Immunol. 1995 Jun;7(6):929-34. doi: 10.1093/intimm/7.6.929.
The increase in autoantibodies with age of both experimental animals and humans has been thought to reflect a shift in the antibody repertoire from foreign to self antigens. In mice, before immunization, the age-associated increase in antibodies reactive with a prototypic autoantigen, bromelain-treated autologous erythrocytes (BrMRBC), reflected a 3-fold increase in serum IgM and the number of IgM-secreting spleen cells in old compared with young mice. However, the percentage of the IgM-secreting spleen cell repertoire reactive with BrMRBC in old mice was actually approximately 50% that in young mice. In contrast, after immunization with sheep erythrocytes (SRBC), old mice showed a 5-fold increase in the percentage of IgM-secreting cells reactive with BrMRBC while young mice showed no significant increase. The converse is true for the percentage of IgM-secreting spleen cells in old mice specific for SBRC, which is 10% the number generated by young mice. The increased autoantibody response of old mice is not, however, linked to their poor response to the nominal antigen. Thus, immunization with phosphorylcholine (PC) conjugated keyhole limpet hemocyanin, an antigen that induces a comparable anti-PC response in old and young mice, also induced more autoantibody forming cells in old than young mice. The increased autoantibody response of old mice after immunization can be accounted for by both an increased number of Ig-secreting spleen cells as well as an increased percentage of the expressed repertoire of IgM-secreting spleen cells that react with autoantigens.(ABSTRACT TRUNCATED AT 250 WORDS)
实验动物和人类体内自身抗体随年龄增长而增加，这被认为反映了抗体库从针对外来抗原向自身抗原的转变。在小鼠中，免疫前，与原型自身抗原——菠萝蛋白酶处理的自体红细胞（BrMRBC）反应的抗体随年龄增长而增加，这反映出老年小鼠血清IgM和分泌IgM的脾细胞数量比幼年小鼠增加了3倍。然而，老年小鼠中与BrMRBC反应的分泌IgM的脾细胞库百分比实际上约为幼年小鼠的50%。相反，在用绵羊红细胞（SRBC）免疫后，老年小鼠中与BrMRBC反应的分泌IgM的细胞百分比增加了5倍，而幼年小鼠则无显著增加。对于老年小鼠中针对SBRC的分泌IgM的脾细胞百分比而言，情况则相反，其数量仅为幼年小鼠产生数量的10%。然而，老年小鼠自身抗体反应的增加与其对名义抗原的较差反应并无关联。因此，用磷酸胆碱（PC）偶联的钥孔戚血蓝蛋白免疫，这种抗原在老年和幼年小鼠中诱导出相当的抗PC反应，同时也在老年小鼠中比幼年小鼠诱导出更多的自身抗体形成细胞。免疫后老年小鼠自身抗体反应的增加可归因于分泌Ig的脾细胞数量增加以及与自身抗原反应的分泌IgM的脾细胞表达库百分比增加。（摘要截选至250字）
