Zaizen Y, Ford E G, Shimada H, Kosi M, Costin G, Atkinson J B
Department of Pediatric Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Eur J Pediatr Surg. 1995 Aug;5(4):226-30. doi: 10.1055/s-2008-1066211.
Systemic growth hormone (GH) markedly improves celiotomy wound strength in protein malnourished (PM) animals. This study was undertaken to analyze the effect of GH as a basis for anatomically understanding. Adult female Spraque-Dawley rats were divided into normally nourished controls, PM and GH-treated PM groups. Protein malnutrition was achieved by feeding 5.5% protein restricted chow every other day for eight weeks before surgery. Controls were fed 23.4% protein chow. All animals were fed 23.4% protein chow postoperatively. Rat-GH was injected subcutaneously twice daily (1.0 mg/day) for three days prior to and five days after 5 cm midline celiotomy. Bursting strength of the wound was measured at 3, 6 and 14 days postoperatively. Histologic wound specimens (hematoxylin and eosin) were obtained from each group. Wound strength of malnourished rats was significantly less than that of normal controls at six days after operation (p < 0.001). With administration of growth hormone, the wound strength was significantly improved. Histologically, there was no difference between groups on day 3. On day 6 the normal control group showed a decrease in the early inflammatory cell infiltrate with concurrent development of granulation tissue and a dense proliferation of fibroblasts. The PM wound showed fatty infiltration, a very narrow band of poorly formed granulation tissue and a sparse fibroblastic proliferation. The GH-treated PM group showed a combination of histologic findings. Fatty infiltration, similar to that in malnourished non-treated animals, was still evident but there was also a dense proliferation of capillary channels and fibroblasts comparable to normal animals.(ABSTRACT TRUNCATED AT 250 WORDS)
全身应用生长激素（GH）可显著提高蛋白质营养不良（PM）动物剖腹手术伤口的强度。本研究旨在分析GH的作用，以便从解剖学角度进行理解。成年雌性Sprague-Dawley大鼠被分为正常营养对照组、PM组和GH治疗的PM组。术前八周，通过每隔一天喂食5.5%蛋白质限制饲料来造成蛋白质营养不良。对照组喂食23.4%蛋白质饲料。所有动物术后均喂食23.4%蛋白质饲料。在5 cm中线剖腹术前3天和术后5天，每天皮下注射两次大鼠GH（1.0 mg/天）。在术后3天、6天和14天测量伤口的破裂强度。从每组获取组织学伤口标本（苏木精和伊红染色）。术后6天，营养不良大鼠的伤口强度明显低于正常对照组（p < 0.001）。使用生长激素后，伤口强度显著改善。组织学上，术后3天各组之间无差异。术后6天，正常对照组早期炎症细胞浸润减少，同时肉芽组织形成，成纤维细胞密集增殖。PM伤口显示脂肪浸润、一条非常窄的形成不良的肉芽组织带和稀疏的成纤维细胞增殖。GH治疗的PM组显示出组织学表现的组合。与未治疗的营养不良动物相似的脂肪浸润仍然明显，但也有与正常动物相当的密集的毛细血管和成纤维细胞增殖。（摘要截断于250字）
