Takano K, Mouri N, Sakurai H, Muto S, Koshizuka K, Tada Y
Second Department of Surgery, Yamanashi Medical University, Japan.
J Pediatr Surg. 1994 Jun;29(6):790-3. doi: 10.1016/0022-3468(94)90372-7.
This study was designed to evaluate the efficacy of human growth hormone (GH) in improving the tensile strength of wounds weakened by chronic protein malnourishment and corticosteroid (CS) administration. Eighty-six female Sprague-Dawley rats, weighing 80 to 100 g, were divided into five groups. Group 1 (control) received 23.4% protein chow for 8 weeks before surgery. Groups 2, 3, 4, and 5 received nonprotein chow on alternate days for the same duration. Groups 3 and 5 received prednisolone (2 mg/kg/d intramuscularly) for 3 weeks preoperatively and for 5 days postoperatively. Groups 4 and 5 were given GH (somatotropin, 1 IU/d) for 5 days postoperatively. All the animals underwent a precise 4-cm midline celiotomy. Wound testing was performed on the sixth postoperative day, after removal of the sutures. The bursting strength (BS, mean +/- SD) for group 1 was 145 +/- 16 mm Hg. The BS for groups 4 (137 +/- 13 mm Hg) and 5 (134 +/- 7 mm Hg) were significantly stronger than those for groups 2 (115 +/- 15 mm Hg) and 3 (91 +/- 16 mm Hg). The authors conclude that postoperative systemic GH restored the wound BS in protein-malnourished animals treated with CS, to the level of the normally nourished controls.
本研究旨在评估人生长激素（GH）对改善因慢性蛋白质营养不良和给予皮质类固醇（CS）而减弱的伤口抗张强度的疗效。86只体重80至100克的雌性斯普拉格-道利大鼠被分为五组。第1组（对照组）在手术前8周给予23.4%蛋白质含量的食物。第2、3、4和5组在相同时间段内隔日给予无蛋白质食物。第3组和第5组在术前3周和术后5天给予泼尼松龙（2毫克/千克/天，肌肉注射）。第4组和第5组在术后5天给予GH（生长激素，1国际单位/天）。所有动物均接受精确的4厘米中线剖腹术。在术后第六天拆除缝线后进行伤口测试。第1组的破裂强度（BS，平均值±标准差）为145±16毫米汞柱。第4组（137±13毫米汞柱）和第5组（134±7毫米汞柱）的BS明显强于第2组（115±15毫米汞柱）和第3组（91±16毫米汞柱）。作者得出结论，术后全身性GH可将接受CS治疗的蛋白质营养不良动物的伤口BS恢复到正常营养对照组的水平。
