Araga S, Kishimoto M, Adachi A, Nakayasu H, Takenaka T, Takahashi K
Division of Neurology, Faculty of Medicine, Tottori University, Yonago, Japan.
Autoimmunity. 1995;20(2):129-34. doi: 10.3109/08916939509001937.
A high frequency of CD5+ B lymphocytes in the peripheral blood of patients with myasthenia gravis (MG) has been reported recently. These results seem to indicate an attractive linkage between CD5+ B lymphocytes and autoantibodies against Acetylcholine receptor in MG. We examined the frequency of CD5+ B cells in 20 patients with MG and 21 normal healthy controls by two-color flow cytometry. However, there were no significant differences in the percentages of CD5+ B lymphocytes between the two groups. We also examined the frequency of CD5+ B lymphocytes in the resected thymus of patients. The frequency of CD5+ B lymphocytes in the thymus was low and similar pattern to that in the peripheral blood. We checked the antibody (Ab) production against the human acetylcholine receptor in either CD5+ B or CD5- B lymphocytes using B lymphoblastoid cell line generated from the lymphocytes of 11 patients with anti-AChR Abs in the sera. Abs against the AChR in the human were mostly produced by CD5- B, not CD5+ B lymphocytes. The anti-AChR Abs (IgG) production of CD5+ B cells and CD5- B cells (mean +/- SD) were 6.8 +/- 2.4 fmol/ml and 18.5 +/- 17.6 fmol/ml, respectively. These results suggest that in MG, the frequencies of the CD5+ B lymphocytes in PBL may be genetic background and that there may be no strong linages between AChR Ab production and CD5+ B lymphocytes.
最近有报道称重症肌无力（MG）患者外周血中CD5⁺B淋巴细胞的频率较高。这些结果似乎表明MG中CD5⁺B淋巴细胞与抗乙酰胆碱受体自身抗体之间存在有吸引力的联系。我们通过双色流式细胞术检测了20例MG患者和21名正常健康对照者中CD5⁺B细胞的频率。然而，两组之间CD5⁺B淋巴细胞的百分比没有显著差异。我们还检测了患者切除胸腺中CD5⁺B淋巴细胞的频率。胸腺中CD5⁺B淋巴细胞的频率较低，且与外周血中的模式相似。我们使用从11例血清中有抗AChR抗体的患者淋巴细胞产生的B淋巴母细胞系，检测了CD5⁺B或CD5⁻B淋巴细胞中针对人乙酰胆碱受体的抗体（Ab）产生情况。针对人AChR的抗体大多由CD5⁻B而非CD5⁺B淋巴细胞产生。CD5⁺B细胞和CD5⁻B细胞抗AChR抗体（IgG）的产生量（平均值±标准差）分别为6.8±2.4 fmol/ml和18.5±17.6 fmol/ml。这些结果表明，在MG中，外周血淋巴细胞（PBL）中CD5⁺B淋巴细胞的频率可能是遗传背景，并且AChR抗体产生与CD5⁺B淋巴细胞之间可能没有紧密联系。
