Vijayachandra K, Palanichelvam K, Veluthambi K
Department of Plant Molecular Biology, School of Biotechnology, Madurai Kamaraj University, India.
Plant Mol Biol. 1995 Oct;29(1):125-33. doi: 10.1007/BF00019124.
For successful transformation of a plant by Agrobacterium tumefaciens it is essential that the explant used in cocultivation has the ability to induce Agrobacterium tumour-inducing (Ti) plasmid virulence (vir) genes. Here we report a significant variation in different tissues of Indica rice (Oryza sativa L. cv. Co43) in their ability to induce Agrobacterium tumefaciens vir genes and T-strand generation, using explants preincubated in liquid Murashige and Skoog (MS) medium. An analysis of rice leaf segments revealed that they neither induced vir genes nor inhibited vir gene induction. Of different parts of rice plants of different ages analysed only scutellum from four-day old rice seedlings induced vir genes and generation of T-strands. We observed that the physical presence of preincubated scutella is required for vir gene induction. Conditioned medium from which preincubated scutella were removed did not induce the vir genes. Scutellum-derived calli, cultured for 25 days on medium containing 2,4-D, also induced virE to an appreciable level. These results suggest that scutellum and scutellum-derived calli may be the most susceptible tissues of rice for Agrobacterium-mediated transformation.
为了通过根癌农杆菌成功转化植物，共培养中使用的外植体必须具备诱导根癌农杆菌致瘤（Ti）质粒毒力（vir）基因的能力。在此，我们报道了籼稻（Oryza sativa L. cv. Co43）不同组织在诱导根癌农杆菌vir基因和T链产生能力方面存在显著差异，使用的外植体是在液体Murashige和Skoog（MS）培养基中预培养的。对水稻叶片切段的分析表明，它们既不诱导vir基因，也不抑制vir基因的诱导。在分析的不同年龄水稻植株的不同部位中，只有四天龄水稻幼苗的盾片能诱导vir基因并产生T链。我们观察到，预培养的盾片的物理存在是诱导vir基因所必需的。去除预培养盾片后的条件培养基不能诱导vir基因。在含有2,4 - D的培养基上培养25天的盾片来源愈伤组织也能将virE诱导到相当水平。这些结果表明，盾片和盾片来源的愈伤组织可能是水稻中对农杆菌介导转化最敏感的组织。
