Wall J G, Plückthun A
University of Zürich, Switzerland.
Curr Opin Biotechnol. 1995 Oct;6(5):507-16. doi: 10.1016/0958-1669(95)80084-0.
Interest continues to increase in the use of folding modulators to overcome problems with heterologous protein folding in Escherichia coli. Currently, this approach, though highly successful with a number of individual proteins, remains a somewhat hit-and-miss affair. Ongoing research directed at unraveling the precise role and specificity of these folding modulators should generate a clearer understanding of the potential and limitations of overexpressing folding catalysts in vivo. This will facilitate the development, in the not too distant future, of a more structured and rational approach to improving the folding of heterologous gene products in E. coli.
人们对于使用折叠调节剂来克服大肠杆菌中异源蛋白质折叠问题的兴趣持续增加。目前，这种方法虽然在许多单个蛋白质上取得了很大成功，但仍然有些碰运气。针对阐明这些折叠调节剂的确切作用和特异性的正在进行的研究，应该会使人们对在体内过表达折叠催化剂的潜力和局限性有更清晰的认识。这将有助于在不久的将来开发出一种更具系统性和合理性的方法，来改善大肠杆菌中异源基因产物的折叠。
