Uusitalo J M, Nevalainen K M, Harkki A M, Knowles J K, Penttilä M E
VTT, Biotechnical Laboratory, Espoo, Finland.
J Biotechnol. 1991 Jan;17(1):35-49. doi: 10.1016/0168-1656(91)90025-q.
The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.
描述了利用纤维素分解丝状真菌里氏木霉生产同源和异源蛋白质的情况。通过基因工程方法构建分泌新型纤维素酶混合物的菌株，在纤维素酶生产方面取得了具有重要生物技术意义的改进。这些改进是通过基因失活和启动子改变实现的。编码主要纤维素酶纤维二糖水解酶I（CBHI）的基因的强且高度可诱导的启动子也已用于在里氏木霉中生产真核异源蛋白质。详细描述了活性小牛凝乳酶的表达和分泌。
