Hippenmeyer P, Highkin M
Monsanto Company, St. Louis, MO 63198.
Biotechnology (N Y). 1993 Sep;11(9):1037-41. doi: 10.1038/nbt0993-1037.
We have engineered mammalian cell lines to produce high levels of heterologous proteins by constructing a cell line that expresses the herpesvirus transactivator, VP16. Subsequent stable transfection with a gene of interest under control of a herpesvirus immediate early promoter led to a rapid isolation of cell lines producing between 1 and 20 micrograms of protein/million cells/24 hours. This high level expression is stable for at least five months.
我们通过构建一种表达疱疹病毒反式激活因子VP16的细胞系，设计出了能够高水平产生异源蛋白的哺乳动物细胞系。随后，将一个受疱疹病毒立即早期启动子控制的目的基因进行稳定转染，从而迅速分离出了每百万细胞每24小时能产生1至20微克蛋白质的细胞系。这种高水平表达至少能稳定五个月。
