Wier P J, Rumberger D
SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939, USA.
Reprod Toxicol. 1995 May-Jun;9(3):327-30. doi: 10.1016/0890-6238(95)00015-3.
A technique for isolation of viable spermatozoa from the rat vas deferens is presented. A single vas deferens from a mature Sprague-Dawley rat contains 35.4 +/- 3.3 million spermatozoa, and a 0.5-cm segment from the distal end of the vas will spontaneously expel 8.96 +/- 1.39 million sperm relatively free of cellular debris when placed into a balanced salt solution. Treatment of rats (n = 5) with alpha-chlorohydrin (100 mg/kg, single dose) significantly reduced, relative to controls (n = 5, water 10 mL/kg), sperm motility by 72%, swimming velocity by 44%, and head displacement motion by 50%, but did not affect the number of sperm in the vas deferens nor sperm recovery. In summary, the isolation of rat sperm from the vas deferens can be achieved with minimum handling in a manner that is not confounded by sperm toxicity.
本文介绍了一种从大鼠输精管中分离活精子的技术。一只成熟的Sprague-Dawley大鼠的单个输精管含有3540±330万个精子，当将输精管远端0.5厘米的片段放入平衡盐溶液中时，它会自发排出896±139万个相对无细胞碎片的精子。相对于对照组（n = 5，10 mL/kg水），用α-氯醇（100 mg/kg，单剂量）处理大鼠（n = 5）可使精子活力显著降低72%，游泳速度降低44%，头部位移运动降低50%，但不影响输精管中的精子数量和精子回收率。总之，以一种不受精子毒性干扰的方式，通过最少的操作即可从大鼠输精管中分离精子。
