Dostal L A, Faber C K, Zandee J
Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan 48105, USA.
Reprod Toxicol. 1996 May-Jun;10(3):231-5. doi: 10.1016/0890-6238(96)00027-5.
Motion parameters were compared in rat sperm isolated from the distal vas deferens and the cauda epididymidis. Motion parameters were also compared in 20 microns and 50 microns deep muCellTM chambers using vas deferens sperm. Video recorded samples were analyzed manually for motility, and analyzed by a computer automated sperm analysis (CASA) system for motility, curvilinear velocity, linearity, mean and maximum amplitude of lateral head displacement (ALH), and beat/cross frequency using two versions of CellSoftTM (Series 3,000 and Series 4,000). Motility, linearity, and beat/cross frequency were not significantly different between sperm from vas deferens and cauda epididymidis, while velocity and ALH values were slightly greater in sperm from vas deferens than from cauda epididymidis. Sperm motility and linearity were not significantly different when analyzed in 20 microns and 50 microns mu CellTM chambers. Velocity and ALH values were slightly greater in 20 microns than in 50 microns chambers, and beat/cross frequency was slightly lower in 20 microns than in 50 microns chambers. Sperm motility was significantly greater when determined manually than when determined with the Series 3,000 but manually determined sperm motility was only slightly greater than motility determined with the Series 4,000. Several sperm motion parameters differed significantly between the Series 3,000 and Series 4,000 (curvilinear velocity, mean and maximum ALH, linearity, and beat/cross frequency) but the relative variability of the systems was comparable. Compared with manual determinations, the Series 3,000 overestimated and the Series 4,000 underestimated the number of cells analyzed for motility. Therefore, differences existed between manual and CellSoft (Series 3,000 and 4,000) analysis of sperm motility and number of cells, and between CellSoft systems in the analysis of sperm motion parameters. However, only minimal differences in sperm motion parameters were observed between the vas deferens and cauda epididymidis, and between 20 microns and 50 microns deep muCell chambers.
对从输精管远端和附睾尾部分离出的大鼠精子的运动参数进行了比较。还使用输精管精子在20微米和50微米深的muCellTM小室中比较了运动参数。对视频记录的样本进行手动分析以检测活力，并使用两个版本的CellSoftTM（3000系列和4000系列）通过计算机自动精子分析（CASA）系统分析活力、曲线速度、直线性、头部横向位移的平均和最大幅度（ALH）以及摆动/交叉频率。输精管精子和附睾尾部精子之间的活力、直线性和摆动/交叉频率没有显著差异，而输精管精子的速度和ALH值略高于附睾尾部精子。在20微米和50微米的muCellTM小室中分析时，精子活力和直线性没有显著差异。20微米小室中的速度和ALH值略高于50微米小室，20微米小室中的摆动/交叉频率略低于50微米小室。手动测定的精子活力显著高于使用3000系列测定的结果，但手动测定的精子活力仅略高于使用4000系列测定的结果。3000系列和4000系列之间的几个精子运动参数存在显著差异（曲线速度、平均和最大ALH、直线性和摆动/交叉频率），但系统的相对变异性相当。与手动测定相比，3000系列高估了用于分析活力的细胞数量，而4000系列低估了该数量。因此，手动和CellSoft（3000系列和4000系列）对精子活力和细胞数量的分析之间，以及CellSoft系统在精子运动参数分析方面存在差异。然而，在输精管和附睾尾部之间，以及20微米和50微米深的muCell小室之间，仅观察到精子运动参数的微小差异。
