Hanagiri T, Igisu H, Shiraishi T, Ikeda M, Yasumoto K
Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Surg Today. 1995;25(6):542-7. doi: 10.1007/BF00311312.
We describe herein a new experimental model in which an isolated rat lung was ventilated with a mixture of 95% nitrogen and 5% carbon dioxide to decrease the oxygen and increase the carbon dioxide in the perfused blood to create and maintain a gas composition similar to that of venous blood. By utilizing this system as a "deoxygenator," pulmonary functions, including gas exchange, could be measured for at least 60 min in isolated and preserved lungs on reperfusion. When the effects of glucose in the flushing and storage solution were examined, 5 mM glucose in the solution resulted in better preservation of the lung, as shown by a higher uptake of oxygen and a lower intratracheal pressure, than when no glucose was given. However, the presence of 50 mM glucose was not beneficial, but rather increased the wet/dry weight ratio of the tissue.
我们在此描述一种新的实验模型，在该模型中，用95%氮气和5%二氧化碳的混合气体对离体大鼠肺进行通气，以降低灌注血液中的氧气含量并增加二氧化碳含量，从而产生并维持类似于静脉血的气体成分。通过将该系统用作“脱氧器”，在再灌注时可对离体保存的肺进行至少60分钟的肺功能测量，包括气体交换。当检测冲洗和保存溶液中葡萄糖的作用时，溶液中5 mM葡萄糖可使肺得到更好的保存，表现为与未给予葡萄糖时相比，氧气摄取量更高且气管内压力更低。然而，50 mM葡萄糖并无益处，反而增加了组织的湿/干重比。
