Hanagiri T, Igisu H, Shiraishi T, Shirakusa T
Second Department of Surgery, School of medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
J UOEH. 1994 Mar 1;16(1):85-9. doi: 10.7888/juoeh.16.85.
We studied the effect of Coenzyme Q10 (CoQ10) on lung preservation using an isolated rat lung reperfusion model. After the lung of a Wistar male rat was flushed with a solution consisting of 40 mM phosphate buffered saline, the heart-lung block was excised and immersed in the same solution at 10 degrees C for 6 hours. In experiment 1, CoQ10 was added to the flushing solution. In experiment 2, CoQ10 was injected i.v. 1 hour before the organ was harvested. After the preservation, both lungs were reperfused and ventilated for 30 minutes. No benefit was noted when CoQ10 was added to the flushing solution. However, when CoQ10 was pretreated one hour before harvesting, the lungs showed significantly better preservation in pulmonary artery pressure, airway pressure, gas exchange function and the wet/dry weight ratio than those of the control group. Thus, appropriate pretreatment of CoQ10 may have a beneficial effect on lung preservation.
我们使用离体大鼠肺再灌注模型研究了辅酶Q10（CoQ10）对肺保存的影响。用含40 mM磷酸盐缓冲盐水的溶液冲洗Wistar雄性大鼠的肺后，切除心肺块并在10℃下将其浸入相同溶液中6小时。在实验1中，将CoQ10添加到冲洗液中。在实验2中，在收获器官前1小时静脉注射CoQ10。保存后，对两侧肺进行再灌注和通气30分钟。当将CoQ10添加到冲洗液中时未观察到益处。然而，当在收获前1小时进行CoQ10预处理时，与对照组相比，肺在肺动脉压、气道压、气体交换功能和湿/干重比方面显示出明显更好的保存效果。因此，CoQ10的适当预处理可能对肺保存有有益作用。
